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高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (1): 110-113.

• Articles • 上一篇    下一篇

Catalytic Characteristics of Lipase Immobilized in CTAB/TX-100 MBG

SONG Shao-fang1* and LUAN Yu-xia2   

  1. 1. College of Chemistry and Material Science, Shandong Agricultural University, Tai’an 271018, P. R. China;
    2. Institute of Pharmaceutics, School of Pharmaceutical Science, Shandong University, Jinan 250100, P. R. China
  • 收稿日期:2009-02-25 修回日期:2009-03-24 出版日期:2010-01-04 发布日期:2010-03-29
  • 通讯作者: SONG Shao-fang. E-mail: ssf@sdau.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No. 20803044) and the Foundation of Key Laboratory of Colloid and Interface Chemistry of Ministry of Education, Shandong University, China(No.200714).

Catalytic Characteristics of Lipase Immobilized in CTAB/TX-100 MBG

SONG Shao-fang1* and LUAN Yu-xia2   

  1. 1. College of Chemistry and Material Science, Shandong Agricultural University, Tai’an 271018, P. R. China;
    2. Institute of Pharmaceutics, School of Pharmaceutical Science, Shandong University, Jinan 250100, P. R. China
  • Received:2009-02-25 Revised:2009-03-24 Online:2010-01-04 Published:2010-03-29
  • Contact: SONG Shao-fang. E-mail: ssf@sdau.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 20803044) and the Foundation of Key Laboratory of Colloid and Interface Chemistry of Ministry of Education, Shandong University, China(No.200714).

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摘要：

CTAB/TX-100 microemulsion-based gel(MBG), which could be soaked in aqueous solution for a long time without mechanical strength and shape changes, was successfully prepared. Lipase immobilized in this gel had a higher activity than that in CTAB MBG when catalyzed the esterification reaction between n-hexanoic acid and n-octanol and its enantioselectivity was slightly higher when catalyzed the stereoselective esterification reaction between racemic ibuprofen and n-octanol. In aqueous solution, lipase in CTAB/TX-100 MBG could catalyze smoothly the hydrolysis reaction of olive oil and could be reused many times without the evident change of MBG mechanical strength although its activity decreased 41.8% after nine-time recycles.

关键词: Microemulsion-based gel(MBG); Immobilized lipase; Activity; Stereoselectivity; Esterification; Hydrolysis

Abstract:

CTAB/TX-100 microemulsion-based gel(MBG), which could be soaked in aqueous solution for a long time without mechanical strength and shape changes, was successfully prepared. Lipase immobilized in this gel had a higher activity than that in CTAB MBG when catalyzed the esterification reaction between n-hexanoic acid and n-octanol and its enantioselectivity was slightly higher when catalyzed the stereoselective esterification reaction between racemic ibuprofen and n-octanol. In aqueous solution, lipase in CTAB/TX-100 MBG could catalyze smoothly the hydrolysis reaction of olive oil and could be reused many times without the evident change of MBG mechanical strength although its activity decreased 41.8% after nine-time recycles.

Key words: Microemulsion-based gel(MBG); Immobilized lipase; Activity; Stereoselectivity; Esterification; Hydrolysis