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高等学校化学研究 ›› 2009, Vol. 25 ›› Issue (4): 443-445.

• Articles • 上一篇    下一篇

Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor

WANG Xue-jiang1*, XIA Si-qing1, ZHAO Jian-fu1, ZHAO Hong-ning1 and RENAULT Nicole Jaffrezic2   

  1. 1. State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, P. R. China;
    2. Laboratoire de Sciences Analytiques(UMR CNRS 5180), Universite’Claude Bernard Lyon 1, Universite’de Lyon, Villeurbanne Cedex 69622, France
  • 收稿日期:2008-09-22 修回日期:2008-10-14 出版日期:2009-07-25 发布日期:2009-10-16
  • 通讯作者: WANG Xue-jiang. E-mail: wangxj@mail.tongji.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.20707014) and the Opening Foundation of the Environmental Engineering Key Discipline, Zhejiang University of Technology, China(No.20080205).

Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor

WANG Xue-jiang1*, XIA Si-qing1, ZHAO Jian-fu1, ZHAO Hong-ning1 and RENAULT Nicole Jaffrezic2   

  1. 1. State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, P. R. China;
    2. Laboratoire de Sciences Analytiques(UMR CNRS 5180), Universite’Claude Bernard Lyon 1, Universite’de Lyon, Villeurbanne Cedex 69622, France
  • Received:2008-09-22 Revised:2008-10-14 Online:2009-07-25 Published:2009-10-16
  • Contact: WANG Xue-jiang. E-mail: wangxj@mail.tongji.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20707014) and the Opening Foundation of the Environmental Engineering Key Discipline, Zhejiang University of Technology, China(No.20080205).

PDF (PC)

Abstract:

A conductometric nitrate biosensor based on methyl viologen/Nafion®/nitrate reductase(NR) interdigitated electrode for heavy metal determination was proposed. The activity of NR(EC 1.6.6.2) from Asper gillusniger(A. niger) was assayed as a function of metal concentration in the presence of Cu2+, Zn2+, Cd2+ and Pb2+. Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions. Detection limits for Cu2+, Zn2+, Cd2+ and Pb2+ were about 0.05, 0.5, 0.1 and 1.0 μmol/L, respectively. The results show that NR activity could not be regained after exposure to Cu2+, but could be partially recovered after exposure to Zn2+, Pb2+ and Cd2+.

Key words: Conductometric biosensor; Heavy metal; Nitrate reductase