Sponge as Scaffolds in Bone and Cartilage Tissue Engineering

doi:10.1007/s40242-024-4135-0

高等学校化学研究 ›› 2024, Vol. 40 ›› Issue (5): 786-797.doi: 10.1007/s40242-024-4135-0

Sponge as Scaffolds in Bone and Cartilage Tissue Engineering

LUO Lei^1,2, GONG Yining², YAN Liang², BU Yazhong³

1. Medical College, Yan'an University, Yan'an 716000, P. R. China;
2. Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, P. R. China;
3. Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, P. R. China

收稿日期:2024-05-27 出版日期:2024-10-01 发布日期:2024-09-26
通讯作者: BU Yazhong,yazhongbu@xjtu.edu.cn;YAN Liang,yanliangdr5583@163.com E-mail:yazhongbu@xjtu.edu.cn;yanliangdr5583@163.com
基金资助:
This work was supported by the National Natural Science Foundation of China (No.52103184),the Natural Science Basic Research Program of Shaanxi Province,China (No.2024JC-YBQN-0874) and the Strengthening Basic Disciplines Plan of Innovation Ability in Xi'an,China (No.22YXYJ0083).

Sponge as Scaffolds in Bone and Cartilage Tissue Engineering

LUO Lei^1,2, GONG Yining², YAN Liang², BU Yazhong³

1. Medical College, Yan'an University, Yan'an 716000, P. R. China;
2. Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, P. R. China;
3. Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, P. R. China

Received:2024-05-27 Online:2024-10-01 Published:2024-09-26
Contact: BU Yazhong,yazhongbu@xjtu.edu.cn;YAN Liang,yanliangdr5583@163.com E-mail:yazhongbu@xjtu.edu.cn;yanliangdr5583@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (No.52103184),the Natural Science Basic Research Program of Shaanxi Province,China (No.2024JC-YBQN-0874) and the Strengthening Basic Disciplines Plan of Innovation Ability in Xi'an,China (No.22YXYJ0083).

摘要/Abstract

摘要： In recent years, tissue scaffolds have emerged as a new therapeutic method for bone and cartilage regeneration, with sponge scaffolds gaining increasing attention. Sponge scaffolds possess several favorable characteristics, including appropriate biodegradability, excellent biocompatibility, good processability, powerful osteogenic ability, a unique porous structure, and adjustable pore size and connectivity. These attributes make sponge scaffolds a promising option for bone and cartilage regeneration. However, to date, there is a lack of comprehensive reviews summarizing the use of sponge scaffolds in this field. In this review, our objective is to provide a comprehensive overview of the research progress and development trends of sponge scaffolds. Firstly, we introduce the various materials used for sponge scaffolds. Secondly, we summarize the different preparation methods employed for creating sponge scaffolds. Subsequently, we discuss the functional additives that can be incorporated into the scaffolds. Finally, we present some special structures that can be achieved in sponge scaffolds. Our aim is for this review to offer valuable insights and serve as a comprehensive resource for the design of sponge scaffolds in cartilage and bone regeneration.

关键词: Bone, Sponge, Scaffold, Tissue regeneration

Abstract: In recent years, tissue scaffolds have emerged as a new therapeutic method for bone and cartilage regeneration, with sponge scaffolds gaining increasing attention. Sponge scaffolds possess several favorable characteristics, including appropriate biodegradability, excellent biocompatibility, good processability, powerful osteogenic ability, a unique porous structure, and adjustable pore size and connectivity. These attributes make sponge scaffolds a promising option for bone and cartilage regeneration. However, to date, there is a lack of comprehensive reviews summarizing the use of sponge scaffolds in this field. In this review, our objective is to provide a comprehensive overview of the research progress and development trends of sponge scaffolds. Firstly, we introduce the various materials used for sponge scaffolds. Secondly, we summarize the different preparation methods employed for creating sponge scaffolds. Subsequently, we discuss the functional additives that can be incorporated into the scaffolds. Finally, we present some special structures that can be achieved in sponge scaffolds. Our aim is for this review to offer valuable insights and serve as a comprehensive resource for the design of sponge scaffolds in cartilage and bone regeneration.

Key words: Bone, Sponge, Scaffold, Tissue regeneration

LUO Lei, GONG Yining, YAN Liang, BU Yazhong. Sponge as Scaffolds in Bone and Cartilage Tissue Engineering[J]. 高等学校化学研究, 2024, 40(5): 786-797.

LUO Lei, GONG Yining, YAN Liang, BU Yazhong. Sponge as Scaffolds in Bone and Cartilage Tissue Engineering[J]. Chemical Research in Chinese Universities, 2024, 40(5): 786-797.

参考文献

[1] Shi C., Wu T., He Y., Zhang Y., Fu D., Pharmacol Ther., 2020, 207, 107473.
[2] Krishnan Y., Grodzinsky A. J., Matrix Biol., 2018, 71/72, 51.
[3] Morgan E. F., Unnikrisnan G. U., Hussein A. I., Annu. Rev. Biomed. Eng., 2018, 20, 119.
[4] Bliddal H., Leeds A. R., Christensen R., Obes. Rev., 2014, 15, 578.
[5] Li M., Yin H., Yan Z., Li H., Wu J., Wang Y., Wei F., Tian G., Ning C., Li H., Gao C., Fu L., Jiang S., Chen M., Sui X., Liu S., Chen Z., Guo Q., Acta Biomater., 2022, 140, 23.
[6] Nauth A., Schemitsch E., Norris B., Nollin Z., Watson J. T., J. Orthop. Trauma, 2018, 32, S7.
[7] Gong Y., Bu Y., Li Y., Hao D., He B., Kong L., Huang W., Gao X., Zhang B., Qu Z., Wang D., Yan L., Front. Bioeng. Biotechnol., 2022, 10, 1058300.
[8] McGovern J. A., Griffin M., Hutmacher D. W., Dis. Model. Mech., 2018, 11, 033084.
[9] Chung C., Burdick J. A., Adv. Drug Delivery Rev., 2008, 60, 243.
[10] Cheng R., Liu L., Xiang Y., Lu Y., Deng L., Zhang H., Santos H. A., Cui W., Biomaterials, 2020, 232, 119706.
[11] Chen H., Han Q., Wang C., Liu Y., Chen B., Wang J., Advanced Drug Delivery Reviews, 2020, 8, 609.
[12] Liu J., Chen H., Wang Y., Li G., Zheng Z., Kaplan D. L., Wang X., Wang X., ACS Biomater. Sci. Eng., 2020, 6, 1641.
[13] Zhu Y., Zhang L., Duan W., Martin-Saldaña S., Li C., Yu H., Feng L., Zhang X., Du B., Li G., Zheng X., Bu Y., Adv. Healthc. Mater., 2023, 12, 2202122.
[14] Zielinska A., Karczewski J., Eder P., Kolanowski T., Szalata M., Wielgus K., Szalata M., Kim D., Shin S. R., Slomski R., Souto E. B., J. Control. Release, 2023, 359, 207.
[15] Mekhail M., Tabrizian M., Adv. Healthcare Mater., 2014, 3, 1529.
[16] Butorina N. N., Payushina O. V., Sheveleva O. N., Novokreshchenova A. N., Domaratskaya E. I., Istranov L. P., Istranova E. V., Bull. Exp. Biol. Med., 2020,169, 162.
[17] Zhang Y., Leng H., Du Z., Huang Y., Liu X., Zhao Z., Zhang X., Cai Q., Yang X., Biomed. Mater., 2020, 15, 065005.
[18] Liu S., Lau C. S., Liang K., Wen F., Teoh S. H., Curr. Opin. Biotechnol., 2022, 74, 92.
[19] Dong C., Lv Y., Polymers (Basel), 2016, 8, 42.
[20] Wu Z., Korntner S. H., Mullen A. M., Skoufos I., Tzora A., Zeugolis D. I., Biomed. Eng. Adv., 2021, 1, 100002.
[21] Cruz M. A., Fernandes K. R., Parisi J. R., Vale G. C. A., Junior S. R. A., Freitas F. R., Sales A. F. S., Fortulan C. A., Peitl O., Zanotto E., Granito R. N., Ribeiro A. M., Renno A. C. M., J. Bone Miner. Metab., 2020, 38, 639.
[22] Wang W., Qin S., He P., Mao W., Chen L., Hua X., Zhang J., Xiong X., Liu Z., Wang P., Meng Q., Dong F., Li A., Chen H., Xu J., Front Cell Dev. Biol., 2021, 9, 682719.
[23] Kim B., Bouklas N., Cohen I., Bonassar L. J., J. Biomech., 2023, 152, 111591.
[24] Toosi S., Naderi-Meshkin H., Kalalinia F., HosseinKhani H., Heirani-Tabasi A., Havakhah S., Nekooei S., Jafarian A. H., Rezaie F., Peivandi M. T., Mesgarani H., Behravan J., J. Mater. Sci.: Mater. Med., 2019, 30, 33.
[25] Been S., Choi J., Cho H., Jeon G., Song J. E., Bucciarelli A., Khang G., J. Tissue Eng. Regen. Med., 2021, 15, 375.
[26] Gowda A.H.J., Bu Y., Kudina O., Krishna K.V., Bohara R.A., Eglin D., Pandit A., Int. J. Biol. Macromol., 2020, 164, 1384.
[27] Fu Q., Ren H., Zheng C., Zhuang C., Wu T., Qin J., Wang Z., Chen Y., Qi N., J. Biomater. Appl., 2018, 33, 477.
[28] Ying Y., Li B., Liu C., Xiong Z., Bai W., Li J., Ma P., J. Biomater. Appl., 2020, 35, 681.
[29] Tsurkan M. V., Voronkina A., Khrunyk Y., Wysokowski M., Petrenko I., Ehrlich H., Carbohydr. Polym., 2021, 252, 117204.
[30] Pina S., Ribeiro V. P., Marques C. F., Maia F. R., Silva T. H., Reis R. L., Oliveira J. M., Materials (Basel), 2019, 12, 1824.
[31] Machałowski T., Idaszek J., Chlanda A., Heljak M., Piasecki A., Święszkowski W., Jesionowski T., Carbohydr. Polym., 2022, 275, 118750.
[32] Rezaei F. S., Sharifianjazi F., Esmaeilkhanian A., Salehi E., Carbohydr. Polym., 2021, 273, 118631.
[33] Ikono R., Vibriani A., Wibowo I., Saputro K. E., Muliawan W., Bachtiar B. M., Mardliyati E., Bachtiar E. W., Rochman N. T., Kagami H., Xianqi L., Nagamura-Inoue T., Tojo A., BMC Res. Notes, 2019, 12, 383.
[34] Serrano-Bello J., Cruz-Maya I., Suaste-Olmos F., Gonzalez-Alva P., Altobelli R., Ambrosio L., Medina L. A., Guarino V., Alvarez-Perez M. A., Front Bioeng. Biotechnol., 2020, 8, 587.
[35] Rockwood D. N., Preda R. C., Yucel T., Wang X., Lovett M. L., Kaplan D. L., Nat. Protoc., 2011, 6, 1612.
[36] Yan R., Chen Y., Gu Y., Tang C., Huang J., Hu Y., Zheng Z., Ran J., Heng B., Chen X., Yin Z., Chen W., Shen W., Ouyang H., J. Tissue Eng. Regen. Med., 2019, 13,156.
[37] Grabska-Zielinska S., Sionkowska A., Carvalho A., Monteiro F. J., Materials (Basel), 2021, 14, 1105.
[38] Carballo C. B., Nakagawa Y., Sekiya I., Rodeo S. A., Clin. Sports Med., 2017, 36, 413.
[39] Utomo D. N., Mahyudin F., Wardhana T. H., Purwati P., Brahmana F., Gusti A. W. R., Int. J. Biomater., 2019, 2019, 8356872.
[40] Monibi F. A., Cook J. L., Tissue Eng. Part B: Rev., 2017, 23, 386.
[41] Ding Y., Zhang W., Sun B., Mo X., Wu J., J. Biomed. Mater. Res., Part A, 2022, 110, 1824.
[42] Arif U., Haider S., Haider A., Khan N., Alghyamah A. A., Jamila N., Khan M. I., Almasry W. A., Kang I. K., Curr. Pharm. Des., 2019, 25, 3608.
[43] Austin M. J., Rosales A. M., Biomater Sci., 2019, 7, 490.
[44] Bueno E. M., Glowacki J., Nat. Rev. Rheumatol., 2009, 5, 685.
[45] Tschon M., Brogini S., Parrilli A., Bertoldi S., Silini A., Parolini O., Fare S., Martini L., Veronesi F., Fini M., Giavaresi G., Biotechnol. Bioeng., 2021, 118, 465.
[46] Rahmanisa S., Prajatelistia E., Wibowo I., Barlian A., Indones. Biomed. J., 2022, 14, 382.
[47] Murugan S., Parcha S. R., J. Mater. Sci. Mater. Med., 2021, 32, 93.
[48] Tziveleka L. A., Sapalidis A., Kikionis S., Aggelidou E., Demiri E., Kritis A., Ioannou E., Roussis V., Materials (Basel), 2020, 13, 1763.
[49] Janik H., Marzec M., Mater. Sci. Eng., C, 2015, 48, 586.
[50] Korbut A., Wlodarczyk M., Rudnicka K., Szwed A., Plocinski P., Biernat M., Tymowicz-Grzyb P., Michalska M., Karska N., Rodziewicz-Motowidlo S., Szustakiewicz K., Int. J. Mol. Sci., 2021, 22, 13589.
[51] Wang K., Yin R., Lu Y., Qiao H., Zhu Q., He J., Zhou W., Zhang H., Tang T., Zhang W., Mater. Sci. Eng., C, 2021, 126, 112185.
[52] Meng C., Liu X., Li R., Malekmohammadi S., Feng Y., Song J., Gong R. H., Li J., Int. J. Biol. Macromol., 2024, 131688.
[53] Liu C., Chu D., Kalantar-Zadeh K., George J., Young H. A., Liu G., Adv. Sci. (Weinh), 2021, 8, e2004433.
[54] Urist M. R., Science, 1965, 150, 893.
[55] Park S. Y., Kim K. H., Kim S., Lee Y. M., Seol Y. J., Pharmaceutics, 2019, 11, 393.
[56] Deininger C., Wagner A., Heimel P., Salzer E., Vila X. M., Weißenbacher N., Grillari J., Redl H., Wichlas F., Freude T., Tempfer H., Teuschl-Woller A. H., Traweger A., Int. J. Mol. Sci., 2021, 23, 283.
[57] Gan Q., Pan H., Zhang W., Yuan Y., Qian J., Liu C., Regen Biomater, 2022, 9, rbac008.
[58] Ferrara N., Adamis A. P., Nat. Rev. Drug Discov., 2016, 15, 385.
[59] Apte R. S., Chen D. S., Ferrara N., Cell, 2019, 176, 1248.
[60] Bakshi R., Hokugo A., Khalil D., Wang L., Shibuya Y., Zhou S., Zhang Z., Rezzadeh K., McClendon M., Stupp S. I., Jarrahy R., Plast. Reconstr. Surg., 2020, 147, 386.
[61] Liu S., Cui F., Ning K., Wang Z., Fu P., Wang D., Xu H., Front. Endocrinol. (Lausanne), 2022, 13, 962968.
[62] Kan T., He Z., Du J., Xu M., Cui J., Han X., Tong D., Li H., Yan M., Yu Z., J. Orthop. Translat., 2022, 37, 37.
[63] Xin X., Wu J., Zheng A., Jiao D., Liu Y., Cao L., Jiang X., Int. J. Nanomedicine, 2019, 14, 1451.
[64] Zhang Y., Qu Z., Zhao Y., Zhang B., Gong Y., Wang X., Gao X., Wang D., Yan L., Curr. Med. Chem., 2024, 31, 2629.
[65] Song J. E., Jeon Y. S., Tian J., Kim W. K., Choi M. J., Carlomagno C., Khang G., Mater. Sci. Eng., C, 2019, 97, 347.
[66] Song J. E., Tian J., Kook Y. J., Thangavelu M., Choi J. H., Khang G., J. Biomed. Mater. Res., Part A, 2019, 108, 784.
[67] Wu L., Wang Y., Xu X., Liu Y., Lin B., Zhang M., Zhang J., Wan S., Yang C., Tan W., Chem. Rev., 2021, 121, 12035.
[68] Wang X., Song X., Li T., Chen J., Cheng G., Yang L., Chen C., Am. J. Sports. Med., 2019, 47, 2316.
[69] Zhou B., Jiang X., Zhou X., Tan W., Luo H., Lei S., Yang Y., Biomater. Res., 2023, 27, 86.
[70] Zhao Q., Ni Y., Wei H., Duan Y., Chen J., Xiao Q., Gao J., Yu Y., Cui Y., Ouyang S., Miron R. J., Zhang Y., Wu C., Periodontol., 2000, 2023, 213.
[71] Ikono R., Li N., Pratama N. H., Vibriani A., Yuniarni D. R., Luthfansyah M., Bachtiar B. M., Bachtiar E. W., Mulia K., Nasikin M., Kagami H., Li X., Mardliyati E., Rochman N. T., Nagamura-Inoue T., Tojo A., Biotechnol. Rep., 2019, 24, e00350.
[72] Lin S.-J., Huang C.-C., Int. J. Mol. Sci., 2022, 23, 6322.
[73] Hoveidaei A. H., Sadat-Shojai M., Mosalamiaghili S., Salarikia S. R., Roghani-shahraki H., Ghaderpanah R., Ersi M. H., Conway J. D., Bone, 2024, 179, 116956.
[74] Yu S., Shu X., Chen L., Wang C., Wang X., Jing J., Yan G., Zhang Y., Wu C., Sci. Rep., 2023, 13, 20168.
[75] Messaoudi O., Henrionnet C., Bourge K., Loeuille D., Gillet P., Pinzano A., Cells, 2020, 10, 2.
[76] Venkataiah V. S., Yahata Y., Kitagawa A., Inagaki M., Kakiuchi Y., Nakano M., Suzuki S., Handa K., Saito M., Cells, 2021, 10, 2687.
[77] Widhiyanto L., Utomo D. N., Perbowo A. P., Hernugrahanto K. D., J. Biomater. Appl., 2020, 35, 422.
[78] Lin C. Y., Int. J. Mol. Sci., 2022, 23, 11377.
[79] Wu Y., Hong J., Jiang G., Li S., Chen S., Chen W., Yan R., Feng G., Cheng Z., Regen. Med., 2019, 14, 753.
[80] Wang C.-Y., Kuo Z.-K., Hsieh M.-K., Ke L.-Y., Chen C.-C., Cheng C.-M., Lai P.-L., Biomed. Mater., 2019, 15, 015005.
[81] Jurczak P., Lach S., Macromol. Biosci., 2023, 23, e2300152.
[82] Santhakumar S., Oyane A., Nakamura M., Yoshino Y., Alruwaili M. K., Miyaji H., Materials (Basel), 2021, 14, 5860.
[83] Nathanael A. J., Oyane A., Nakamura M., Sakamaki I., Nishida E., Kanemoto Y., Miyaji H., ACS Appl. Mater. Interfaces, 2017, 9, 22185.
[84] Han X., Saiding Q., Cai X., Xiao Y., Wang P., Cai Z., Gong X., Gong W., Zhang X., Cui W., Nano-Micro Lett., 2023, 15, 239.
[85] Amann E., Amirall A., Franco A. R., Poh P. S. P., Sola Dueñas F. J., Fuentes Estévez G., Leonor I. B., Reis R. L., van Griensven M., Balmayor E. R., Adv. Healthcare Mater., 2021, 10, e2001692.
[86] Baudequin T., Agnes C., Tabrizian M., Carbohydr. Polym., 2021, 256, 117499.
[87] Xie Y., Lee K., Wang X., Yoshitomi T., Kawazoe N., Yang Y., Chen G., J. Mater. Chem. B, 2021, 9, 8491.
[88] Diaz F., Forsyth N., Boccaccini A. R., Adv. Healthcare Mater., 2023, 12, e2203205.
[89] Sturtivant A., Callanan A., Biomed. Phys. Eng. Express, 2020, 6, 055016.
[90] Lesage C., Lafont M., Guihard P., Weiss P., Guicheux J., Delplace V., Adv. Sci. (Weinh), 2022, 9, e2200050.
[91] Huang W., Wu X., Zhao Y., Liu Y., Zhang B., Qiao M., Zhu Z., Zhao Z., ACS Appl. Mater. Interfaces, 2023, 15, 2639.
[92] Baudequin T., Tabrizian M., Adv. Healthcare Mater., 2018, 7, e1700734.
[93] Wu R., Li Y., Shen M., Yang X., Zhang L., Ke X., Yang G., Gao C., Gou Z., Xu S., Bioact. Mater., 2021, 6, 1242.
[94] Lopes D., Martins-Cruz C., Oliveira M. B., Mano J. F., Biomaterials, 2018, 185, 240.

Sponge as Scaffolds in Bone and Cartilage Tissue Engineering

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