PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

doi:10.1007/s40242-023-3080-7

高等学校化学研究 ›› 2023, Vol. 39 ›› Issue (6): 1023-1030.doi: 10.1007/s40242-023-3080-7

PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

ZHENG Zhiwen^1,2,3,4, DAI Xin^1,2, LI Xueyang^1,2, and DU Chang^1,2,3,4

1. Department of Biomaterials, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, P.R. China;
2. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P.R. China;
3. NMPA Key Laboratory for Research and Evaluation of Innovative Biomaterials for Medical Devices, South China University of Technology, Guangzhou 510006, P.R. China;
4. Key Laboratory of Biomedical Materials and Engineering, Ministry of Education, South China University of Technology, Guangzhou 510006, P.R. China

收稿日期:2023-03-27 出版日期:2023-12-01 发布日期:2023-11-18
通讯作者: DU Chang E-mail:duchang@scut.edu.cn
基金资助:
This work was supported by the Key Technologies Research and Development Program of China (No.2017YFC1105000), the Natural Science Foundation of Guangdong Province of China (No.2018A030310374), the Guangdong Medical Research Foundation of China (No.2022YDZ09), the Fund of Guangzhou Science, Technology and Innovation Commission of China (No.202102080430) and the Fund of Higher Education Discipline Innovation Project of China (No.B13039).

PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

ZHENG Zhiwen^1,2,3,4, DAI Xin^1,2, LI Xueyang^1,2, and DU Chang^1,2,3,4

1. Department of Biomaterials, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, P.R. China;
2. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P.R. China;
3. NMPA Key Laboratory for Research and Evaluation of Innovative Biomaterials for Medical Devices, South China University of Technology, Guangzhou 510006, P.R. China;
4. Key Laboratory of Biomedical Materials and Engineering, Ministry of Education, South China University of Technology, Guangzhou 510006, P.R. China

Received:2023-03-27 Online:2023-12-01 Published:2023-11-18
Contact: DU Chang E-mail:duchang@scut.edu.cn
Supported by:
This work was supported by the Key Technologies Research and Development Program of China (No.2017YFC1105000), the Natural Science Foundation of Guangdong Province of China (No.2018A030310374), the Guangdong Medical Research Foundation of China (No.2022YDZ09), the Fund of Guangzhou Science, Technology and Innovation Commission of China (No.202102080430) and the Fund of Higher Education Discipline Innovation Project of China (No.B13039).

摘要/Abstract

摘要： More and more concerns about health bring the increasing demand for blood contact tissue engineering alternatives. In this paper, nanoparticles of poly(lactic- co-glycolic acid)/polyethyleneimine mixed with recombinant hirudin (rHNPs) were prepared by a double emulsion solvent volatilization method, which were then loaded onto the polycaprolactone (PCL) with polydopamine (PDA) coating to form the composite nanofibers of PCL/PDA/rHNPs. The hydrophilicity and mechanical properties of the composite nanofibers were improved significantly compared with pure PCL. The morphology kept almost unchanged after 30 d of degradation in phosphate buffer saline (PBS). The anticoagulant molecule of hirudin could be gradually released from the composite scaffolds through the degradation of rHNPs in vitro. When the concentration of rHNPs suspension was 5.0 mg/mL, the composite nanofibers could better promote the growth and proliferation of human umbilical vein endothelial cells (HUVECs). The anticoagulant ability of the composite nanofibers was also significantly improved in comparison with that of pure PCL. The design of controlled release anticoagulant materials would alleviate the sudden release of simple fixed hirudin, which could also provide a new idea for the development of novel blood contact materials.

关键词: Recombinant hirudin, Anticoagulation, Drug release, Composite nanofiber

Abstract: More and more concerns about health bring the increasing demand for blood contact tissue engineering alternatives. In this paper, nanoparticles of poly(lactic- co-glycolic acid)/polyethyleneimine mixed with recombinant hirudin (rHNPs) were prepared by a double emulsion solvent volatilization method, which were then loaded onto the polycaprolactone (PCL) with polydopamine (PDA) coating to form the composite nanofibers of PCL/PDA/rHNPs. The hydrophilicity and mechanical properties of the composite nanofibers were improved significantly compared with pure PCL. The morphology kept almost unchanged after 30 d of degradation in phosphate buffer saline (PBS). The anticoagulant molecule of hirudin could be gradually released from the composite scaffolds through the degradation of rHNPs in vitro. When the concentration of rHNPs suspension was 5.0 mg/mL, the composite nanofibers could better promote the growth and proliferation of human umbilical vein endothelial cells (HUVECs). The anticoagulant ability of the composite nanofibers was also significantly improved in comparison with that of pure PCL. The design of controlled release anticoagulant materials would alleviate the sudden release of simple fixed hirudin, which could also provide a new idea for the development of novel blood contact materials.

Key words: Recombinant hirudin, Anticoagulation, Drug release, Composite nanofiber

ZHENG Zhiwen, DAI Xin, LI Xueyang, and DU Chang. PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect[J]. 高等学校化学研究, 2023, 39(6): 1023-1030.

ZHENG Zhiwen, DAI Xin, LI Xueyang, and DU Chang. PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect[J]. Chemical Research in Chinese Universities, 2023, 39(6): 1023-1030.

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PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

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