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高等学校化学研究 ›› 2023, Vol. 39 ›› Issue (3): 415-424.doi: 10.1007/s40242-023-3019-z

• Article • 上一篇    下一篇

Assessing the Adverse Impacts of PM2.5 on Olfactory System Using an Air-liquid Interface Culture Model of Primary Olfactory Epithelial Cells

Huan Wang1, Ting Xu1,2, Sheng Wei1, Miao Cao1, Daqiang Yin1,2   

  1. 1. Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China;
    2. Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China
  • 收稿日期:2023-01-29 发布日期:2023-05-25
  • 通讯作者: Ting Xu, Daqiang Yin E-mail:xuting@tongji.edu.cn;yindq@tongji.edu.cn
  • 基金资助:
    This work was supported by the National Natural Science Foundation of China (Nos.22076146, 92043302).

Assessing the Adverse Impacts of PM2.5 on Olfactory System Using an Air-liquid Interface Culture Model of Primary Olfactory Epithelial Cells

WANG Huan, XU Ting, WEI Sheng, CAO Miao, YIN Daqiang   

  1. 1. Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China;
    2. Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China
  • Received:2023-01-29 Published:2023-05-25
  • Contact: Ting Xu, Daqiang Yin E-mail:xuting@tongji.edu.cn;yindq@tongji.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (Nos.22076146, 92043302).

摘要： The air-liquid interface(ALI) culture is a kind of recently developed system, which has proved its availability in simulating the biology of respiratory tract epithelial tissues. In this study, an ALI-based mouse primary olfactory epithelial cell(OEC) model was established to perform the exposure of PM2.5 (PM=particulate matter) collected from Dianshan Lake(Shanghai) and Wangdu(Hebei). The results showed that PM2.5 in both regions caused a decrease in cell viability in a dose-dependent manner. The 0.5 and 5 μg/cm2(around ambient concentrations) of PM2.5 disrupted OEC membrane integrity and produced oxidative stress with elevated indicators of malondialdehyde(MDA) and reactive oxygen species(ROS). In transcriptomic sequencing, the terms concerning inflammatory cytokines and second messenger cyclic adenosine-3′,5′-monophoshate(cAMP) were enriched in two treatments. The cytokine array showed the levels of some cytokines were altered, although inflammatory responses may not remarkably occur. Meanwhile, PM2.5 disturbed cAMP contents and key genes in the cAMP signaling pathway. The effects of PM2.5 of both regions were largely consistent, while Wangdu samples caused more ROS and Dianshan Lake samples tended to induce inflammatory injury. Thus, with the application of a novel ALI-based in vitro OEC model, our study demonstrated that ambient PM2.5 has the ability to threaten the physiologies and functions of the olfactory system.

关键词: Air-liquid interface, Olfactory epithelial cell, PM2.5, Inflammatory cytokines, Cyclic adenosine-3′,5′-monophoshate (cAMP)

Abstract: The air-liquid interface(ALI) culture is a kind of recently developed system, which has proved its availability in simulating the biology of respiratory tract epithelial tissues. In this study, an ALI-based mouse primary olfactory epithelial cell(OEC) model was established to perform the exposure of PM2.5 (PM=particulate matter) collected from Dianshan Lake(Shanghai) and Wangdu(Hebei). The results showed that PM2.5 in both regions caused a decrease in cell viability in a dose-dependent manner. The 0.5 and 5 μg/cm2(around ambient concentrations) of PM2.5 disrupted OEC membrane integrity and produced oxidative stress with elevated indicators of malondialdehyde(MDA) and reactive oxygen species(ROS). In transcriptomic sequencing, the terms concerning inflammatory cytokines and second messenger cyclic adenosine-3′,5′-monophoshate(cAMP) were enriched in two treatments. The cytokine array showed the levels of some cytokines were altered, although inflammatory responses may not remarkably occur. Meanwhile, PM2.5 disturbed cAMP contents and key genes in the cAMP signaling pathway. The effects of PM2.5 of both regions were largely consistent, while Wangdu samples caused more ROS and Dianshan Lake samples tended to induce inflammatory injury. Thus, with the application of a novel ALI-based in vitro OEC model, our study demonstrated that ambient PM2.5 has the ability to threaten the physiologies and functions of the olfactory system.

Key words: Air-liquid interface, Olfactory epithelial cell, PM2.5, Inflammatory cytokines, Cyclic adenosine-3′,5′-monophoshate (cAMP)