Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage

doi:10.1007/s40242-018-7255-6

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (1): 33-38.doi: 10.1007/s40242-018-7255-6

Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage

ZHAO Ying, FU Zhijing, CHEN Xiaofei, ZHANG Guoyan

College of Chemistry, Jilin University, Changchun 130012, P. R. China

收稿日期:2017-07-31 出版日期:2018-02-01 发布日期:2018-01-20
通讯作者: ZHANG Guoyan,E-mail:guoyan@jlu.edu.cn E-mail:guoyan@jlu.edu.cn
基金资助:
Supported by the Project of Green Treatment Technology of Acidic Mine Drainage of Wanguo Gold Co., Ltd. of Jilin Pro- vince, China(No.3R113AL51412).

Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage

ZHAO Ying, FU Zhijing, CHEN Xiaofei, ZHANG Guoyan

College of Chemistry, Jilin University, Changchun 130012, P. R. China

Received:2017-07-31 Online:2018-02-01 Published:2018-01-20
Contact: ZHANG Guoyan,E-mail:guoyan@jlu.edu.cn E-mail:guoyan@jlu.edu.cn
Supported by:
Supported by the Project of Green Treatment Technology of Acidic Mine Drainage of Wanguo Gold Co., Ltd. of Jilin Pro- vince, China(No.3R113AL51412).

摘要/Abstract

摘要： Acidic mine drainage(AMD) containing acidity and a broad range of heavy metal ions is classified as hazardous, and must be properly treated. The removal mechanism of heavy metal ions in acidic mine drainage contai- ning Cu²⁺, Fe²⁺, and Zn²⁺with biological method was studied here. Using 20 mmol/L ethanol as carbon source, Desulfovibrio marrakechensis,one of sulfate reducing bacteria(SRB) species, grew best at 35 ℃ and pH=6.72 with concentrations of 10, 55 and 32 mg/L for Cu²⁺, Fe²⁺ and Zn²⁺, respectively. The removal efficiency for each ion mentioned above was 99.99%, 87.64% and 99.88%, respectively. The mineralogy and surface chemistry of precipitates were studied by means of energy dispersive spectrometer(EDS), X-ray photoelectron spectroscopy(XPS), X-ray diffraction(XRD) combined with control tests. The experimental results demonstrate that the removal mechanism of heavy metal ions by Desulfovibrio marrakechensis is comprehensive function of chemical precipitation, adsorption and bioprecipitation. The biogenic iron sulfide solid was characterized as greigite(Fe₃S₄), while the zinc sulfide solid was characterized as sphalerite(ZnS).

关键词: Acid mine drainage, Sulfate reducing bacteria, Heavy metal ion, Precipitate, Removal mechanism

Abstract: Acidic mine drainage(AMD) containing acidity and a broad range of heavy metal ions is classified as hazardous, and must be properly treated. The removal mechanism of heavy metal ions in acidic mine drainage contai- ning Cu²⁺, Fe²⁺, and Zn²⁺with biological method was studied here. Using 20 mmol/L ethanol as carbon source, Desulfovibrio marrakechensis,one of sulfate reducing bacteria(SRB) species, grew best at 35 ℃ and pH=6.72 with concentrations of 10, 55 and 32 mg/L for Cu²⁺, Fe²⁺ and Zn²⁺, respectively. The removal efficiency for each ion mentioned above was 99.99%, 87.64% and 99.88%, respectively. The mineralogy and surface chemistry of precipitates were studied by means of energy dispersive spectrometer(EDS), X-ray photoelectron spectroscopy(XPS), X-ray diffraction(XRD) combined with control tests. The experimental results demonstrate that the removal mechanism of heavy metal ions by Desulfovibrio marrakechensis is comprehensive function of chemical precipitation, adsorption and bioprecipitation. The biogenic iron sulfide solid was characterized as greigite(Fe₃S₄), while the zinc sulfide solid was characterized as sphalerite(ZnS).

Key words: Acid mine drainage, Sulfate reducing bacteria, Heavy metal ion, Precipitate, Removal mechanism

ZHAO Ying, FU Zhijing, CHEN Xiaofei, ZHANG Guoyan. Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage[J]. 高等学校化学研究, 2018, 34(1): 33-38.

ZHAO Ying, FU Zhijing, CHEN Xiaofei, ZHANG Guoyan. Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage[J]. Chemical Research in Chinese Universities, 2018, 34(1): 33-38.

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Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage

Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions inAcidic Mine Drainage

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