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高等学校化学研究 ›› 2013, Vol. 29 ›› Issue (4): 724-729.doi: 10.1007/s40242-013-3078-7

• Articles • 上一篇    下一篇

Extraction of Geniposide and Its Application in Anesthesiology

XU Dan1, DONG Yu-xiang1, FENG Wei2, GE Hong-yan1, LI You-tian1, JIANG Yang2, WANG Li-jie1, SONG Da-qian3   

  1. 1. The First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    3. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • 收稿日期:2013-03-01 修回日期:2013-03-25 出版日期:2013-08-01 发布日期:2013-07-15
  • 通讯作者: LI You-tian, JIANG Yang E-mail:liyoutian9@gmail.com;jy7555@163.com
  • 基金资助:

    Supported by the Natural Science Foundation of Jilin Province, China(No.20125059).

Extraction of Geniposide and Its Application in Anesthesiology

XU Dan1, DONG Yu-xiang1, FENG Wei2, GE Hong-yan1, LI You-tian1, JIANG Yang2, WANG Li-jie1, SONG Da-qian3   

  1. 1. The First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    3. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • Received:2013-03-01 Revised:2013-03-25 Online:2013-08-01 Published:2013-07-15
  • Contact: LI You-tian, JIANG Yang E-mail:liyoutian9@gmail.com;jy7555@163.com
  • Supported by:

    Supported by the Natural Science Foundation of Jilin Province, China(No.20125059).

被引次数

1

摘要：

We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other for the efficiency of geniposide extraction from Gardenia fruit. Extraction with 80% ethanol under reflux was found to give the highest yield of geniposide. Orthogonal experiment was conducted to optimize the reflux conditions and the optimal conditions are a mass(of Gardenia jasminoides Ellis powder) to volume(of solvent, 80% ethanol) ratio of 1:7 and reflux twice for 60 min each. The geniposide obtained by the reflux method was tested on the rats with chronic constriction injury(CCI) for pain relief. The results show that geniposide can enhance pain threshold and relieve pain via repressing tumor necrosis factor-α(TNF-α) expression in either ipsilateral or contralateral dorsal root ganglion(DRG) cells. These results suggest that geniposide treatement may be an effective clinical method to relieve pain based on the mechanism of molecular biology at DRG due to nerve injury.

关键词: Geniposide, Reflux method, Orthogonal experiment, Neuropathic pain

Abstract:

We tried to develop an efficient strategy to isolate geniposide from Gardenia fruit and use it to treat chronic pain. Eight extraction solvents and three extraction methods were respectively compared with each other for the efficiency of geniposide extraction from Gardenia fruit. Extraction with 80% ethanol under reflux was found to give the highest yield of geniposide. Orthogonal experiment was conducted to optimize the reflux conditions and the optimal conditions are a mass(of Gardenia jasminoides Ellis powder) to volume(of solvent, 80% ethanol) ratio of 1:7 and reflux twice for 60 min each. The geniposide obtained by the reflux method was tested on the rats with chronic constriction injury(CCI) for pain relief. The results show that geniposide can enhance pain threshold and relieve pain via repressing tumor necrosis factor-α(TNF-α) expression in either ipsilateral or contralateral dorsal root ganglion(DRG) cells. These results suggest that geniposide treatement may be an effective clinical method to relieve pain based on the mechanism of molecular biology at DRG due to nerve injury.

Key words: Geniposide, Reflux method, Orthogonal experiment, Neuropathic pain