[an error occurred while processing this directive]

高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (3): 361-365.

• Articles • 上一篇    下一篇

Purification Technology and Antimicrobial Activity Analysis of Antimicrobial Peptide from Ovotransferrin

ZHANG Tie-hua, ZHENG Jian, YE Hai-qing, YU Ya-li, ZHAO Ping and LIU Jing-bo*   

  1. College of Light Industry and Economics & Management,Jilin University, Changchun 130062, P. R. China
  • 收稿日期:2010-12-01 修回日期:2011-02-22 出版日期:2011-05-25 发布日期:2011-04-29
  • 通讯作者: LIU Jing-bo E-mail:ljb168@sohu.com
  • 基金资助:

    Supported by the Applied Basic Fund of Jilin Provincial Science and Technology Department(No.20070572) and the Frontiers of Science of Jilin University and Innovation Fund of Interdisciplinary, China(No.200903224).

Purification Technology and Antimicrobial Activity Analysis of Antimicrobial Peptide from Ovotransferrin

ZHANG Tie-hua, ZHENG Jian, YE Hai-qing, YU Ya-li, ZHAO Ping and LIU Jing-bo*   

  1. College of Light Industry and Economics & Management,Jilin University, Changchun 130062, P. R. China
  • Received:2010-12-01 Revised:2011-02-22 Online:2011-05-25 Published:2011-04-29
  • Contact: LIU Jing-bo E-mail:ljb168@sohu.com
  • Supported by:

    Supported by the Applied Basic Fund of Jilin Provincial Science and Technology Department(No.20070572) and the Frontiers of Science of Jilin University and Innovation Fund of Interdisciplinary, China(No.200903224).

PDF (PC)

摘要： Antibacterial peptides mixture purified from Ovotransferrin by pepsin digest was used as the raw material. Peptide sections with good antibacterial activity were determined after bacteriostasis experiments, its molecular weight and amino acid composition were analyzed. The results of experiments indicate that with Sephadex G-50 and distilled water as mobile phase, detection wavelength 220 nm, flow rate 1.5 mL/min, sample density 0.2 g/mL, and volume 0.2 mL are the optimal conditions. Bacteriostasis experiments of the fraction of purified peaks were carried out and the result was: peak 1>peak 3>peak 2; the molecular weight of peak 1 was about 3015 by high performance liquid chromatography; active peptide possessed positive charges by amino acid analysis, its cationic characteristics are in accordance with the nature of antimicrobial peptides.

关键词: Ovotransferrin, Purification, Molecular weight, Amino acid

Abstract: Antibacterial peptides mixture purified from Ovotransferrin by pepsin digest was used as the raw material. Peptide sections with good antibacterial activity were determined after bacteriostasis experiments, its molecular weight and amino acid composition were analyzed. The results of experiments indicate that with Sephadex G-50 and distilled water as mobile phase, detection wavelength 220 nm, flow rate 1.5 mL/min, sample density 0.2 g/mL, and volume 0.2 mL are the optimal conditions. Bacteriostasis experiments of the fraction of purified peaks were carried out and the result was: peak 1>peak 3>peak 2; the molecular weight of peak 1 was about 3015 by high performance liquid chromatography; active peptide possessed positive charges by amino acid analysis, its cationic characteristics are in accordance with the nature of antimicrobial peptides.

Key words: Ovotransferrin, Purification, Molecular weight, Amino acid