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高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (3): 406-408.

• Articles • 上一篇    下一篇

Altered Taurine Release in Hypothalamus of Aquaporin-4 Knockout Mice

ZHANG Hong-guo, XIAO Ying-hong, YANG Long-fei, SU Wei-heng, GUAN Xin-gang, WANG Yu, FENG Xue-chao and MA Tong-hui*   

  1. Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China
  • 收稿日期:2010-02-02 修回日期:2010-03-10 出版日期:2010-05-25 发布日期:2010-07-27
  • 通讯作者: MA Tong-hui. E-mail: math108@gmail.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(Nos.30670477 and 30770493).

Altered Taurine Release in Hypothalamus of Aquaporin-4 Knockout Mice

ZHANG Hong-guo, XIAO Ying-hong, YANG Long-fei, SU Wei-heng, GUAN Xin-gang, WANG Yu, FENG Xue-chao and MA Tong-hui*   

  1. Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China
  • Received:2010-02-02 Revised:2010-03-10 Online:2010-05-25 Published:2010-07-27
  • Contact: MA Tong-hui. E-mail: math108@gmail.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30670477 and 30770493).

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摘要：

Taurine is concentrated in glial cells in the hypothalamus and released in an osmo-dependent manner through volume-sensitive anion channels. Released taurine acts on glycine receptors on vasopressin neurons to control vasopressin secretion. Water channel AQP4 is abundant in astrocytes in osmosensory areas such as the supraoptic nucleus of hypothalamus. An HPLC-based method was established to quantify taurine release from isolated hypothalamus tissues in wildtype and AQP4 knockout mice. Under the basal condition, there was no difference in taurine release from AQP4+/+ and AQP4-/- hypothalamuses. Taurine release from AQP4-/- hypothalamus under hypoosmotic stimulation was significantly lower than that from AQP4+/+ mice. AQP4 expression in the glial cells of the hypothalamus may play an important role in osmoregulation of taurine release and subsequent vasopressin secretion.

关键词: Aquaporin-4; Taurine; Hypothalamus; HPLC; Precolumn derivatization

Abstract:

Taurine is concentrated in glial cells in the hypothalamus and released in an osmo-dependent manner through volume-sensitive anion channels. Released taurine acts on glycine receptors on vasopressin neurons to control vasopressin secretion. Water channel AQP4 is abundant in astrocytes in osmosensory areas such as the supraoptic nucleus of hypothalamus. An HPLC-based method was established to quantify taurine release from isolated hypothalamus tissues in wildtype and AQP4 knockout mice. Under the basal condition, there was no difference in taurine release from AQP4+/+ and AQP4-/- hypothalamuses. Taurine release from AQP4-/- hypothalamus under hypoosmotic stimulation was significantly lower than that from AQP4+/+ mice. AQP4 expression in the glial cells of the hypothalamus may play an important role in osmoregulation of taurine release and subsequent vasopressin secretion.

Key words: Aquaporin-4; Taurine; Hypothalamus; HPLC; Precolumn derivatization