高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (3): 376-379.
Determination of Amino Acids by Ru(bpy)32+ Cathodic Electrochemiluminescence with High Sensitivity
ZHOU Xiao-guang1,2, YUAN Bai-qing2, ZHANG Lei3, LIU Yang2, HAN Dong-xue2 and YOU Tian-yan2*
- 1. Department of Chemistry and Chemical Engineering, College of Science, Northeast Forestry University, Harbin 150040, P. R. China;
2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
3. College of Chemistry, Jilin University, Changchun 130021, P. R. China
Determination of Amino Acids by Ru(bpy)32+ Cathodic Electrochemiluminescence with High Sensitivity
ZHOU Xiao-guang1,2, YUAN Bai-qing2, ZHANG Lei3, LIU Yang2, HAN Dong-xue2 and YOU Tian-yan2*
- 1. Department of Chemistry and Chemical Engineering, College of Science, Northeast Forestry University, Harbin 150040, P. R. China;
2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
3. College of Chemistry, Jilin University, Changchun 130021, P. R. China
摘要：
The unique cathodic electrochemiluminescence(ECL) emission of Ru(bpy)32+(bpy=2,2′-bipyridine) was observed via Nafion film at Au electrode[Au/Nafion/Ru(bpy)32+] at about 0.20 V(vs. Ag/AgCl) and applied to the determination of several amino acids without prior derivatization with high sensitivity. The cathodic electrochemiluminescence(ECL) exhibits the detection limits and linear ranges of several amino acids comparable to or better than those of capillary electrophoresis with conventional ECL detection method(at 1.10—1.20 V vs. Ag/AgCl) based on precolumn derivatization. The results suggest that the cathodic ECL is promising for the detection of amino acids in bioanalysis.