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高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (2): 230-234.

• Articles • 上一篇    下一篇

Total Fractionation and Analysis of Polysaccharides from Leaves of Panax ginseng C. A. Meyer

NI Xiu-zhen1,2, WANG Bing-qing1, ZHI Yuan1, WEI Ning-ning1, ZHANG Xu1, LI Shan-shan1, TAI Gui-hua1, ZHOU Yi-fa1* and ZHAO Ji-ming2*   

  1. 1. School of Life Sciences, Northeast Normal University, Changchun 130024, P. R. China;
    2. School of Life Sciences, Changchun Normal University, Changchun 130032, P. R. China
  • 收稿日期:2009-04-20 修回日期:2009-05-18 出版日期:2010-03-25 发布日期:2010-05-25
  • 通讯作者: ZHOU Yi-fa. E-mail: zhouyf383@nenu.edu.cn; ZHAO Ji-ming. E-mail: zmj@263.net
  • 基金资助:

    Supported by the National Natural Science Foundation of China(Nos.30570417 and 30973857), the Natural Science Foundation of Jilin Province(No.20070710), the Natural Science Foundation of Changchun, China(Nos.2009081 and 09SF03).

Total Fractionation and Analysis of Polysaccharides from Leaves of Panax ginseng C. A. Meyer

NI Xiu-zhen1,2, WANG Bing-qing1, ZHI Yuan1, WEI Ning-ning1, ZHANG Xu1, LI Shan-shan1, TAI Gui-hua1, ZHOU Yi-fa1* and ZHAO Ji-ming2*   

  1. 1. School of Life Sciences, Northeast Normal University, Changchun 130024, P. R. China;
    2. School of Life Sciences, Changchun Normal University, Changchun 130032, P. R. China
  • Received:2009-04-20 Revised:2009-05-18 Online:2010-03-25 Published:2010-05-25
  • Contact: ZHOU Yi-fa. E-mail: zhouyf383@nenu.edu.cn; ZHAO Ji-ming. E-mail: zmj@263.net
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30570417 and 30973857), the Natural Science Foundation of Jilin Province(No.20070710), the Natural Science Foundation of Changchun, China(Nos.2009081 and 09SF03).

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摘要：

The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction(GLPN-1), six acidic fractions(GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3) by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The results show that GLPN-1 was mainly composed of Gal(38.1%) and Ara(33.6%) and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1:1, 1:2 and 1:2.7, respectively, indicating that they belong to type-I rhamnogalacturonan (RG-I)-rich pectin. GLPA-1c was mainly composed of GalA(78%), belonging to homogalacturonan(HG)-rich pectin. GLPA-3 might be a glycoprotein.

关键词: Ginseng leave; Polysaccharide; Fractionation; RG-I domain

Abstract:

The water-soluble polysaccharides isolated from the leaves of Panax ginseng C. A. Meyer were totally fractionated into one neutral fraction(GLPN-1), six acidic fractions(GLPN-2, GLPA-1a, GLPA-1b, GLPA-1c, GLPA-2 and GLPA-3) by a combination of ethanol precipitation, ion-exchange and gel permeation chromatography. All the fractions were analyzed by determining monosaccharide composition, molecular weight distribution. The results show that GLPN-1 was mainly composed of Gal(38.1%) and Ara(33.6%) and presumed to be an arabinogalactan. GLPA-1a, GLPA-1b and GLPA-2 mainly contained Gal, Ara, Rha and GalA. The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%. The molar ratios of Rha/GalA in GLPA-1a, GLPA-1b and GLPA-2 were 1:1, 1:2 and 1:2.7, respectively, indicating that they belong to type-I rhamnogalacturonan (RG-I)-rich pectin. GLPA-1c was mainly composed of GalA(78%), belonging to homogalacturonan(HG)-rich pectin. GLPA-3 might be a glycoprotein.

Key words: Ginseng leave; Polysaccharide; Fractionation; RG-I domain