[an error occurred while processing this directive]

高等学校化学研究 ›› 2009, Vol. 25 ›› Issue (6): 891-894.

• Articles • 上一篇    下一篇

Preparation of Adenovirus 5 E1A Polyclonal Antibody

SHEN Qiang1,2, MENG Fan-kai2, YU Yin1, ZHANG Yu1 and RUO Qi1*   

  1. 1. Department of Neurosurgery, First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Department of Neurosurgery, the People’s Hospital of Jilin Province, Changchun 130021, P. R. China
  • 收稿日期:2008-12-01 修回日期:2009-02-02 出版日期:2009-11-25 发布日期:2010-01-25
  • 通讯作者: RUO Qi. E-mail: liluoqi@163.com

Preparation of Adenovirus 5 E1A Polyclonal Antibody

SHEN Qiang1,2, MENG Fan-kai2, YU Yin1, ZHANG Yu1 and RUO Qi1*   

  1. 1. Department of Neurosurgery, First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Department of Neurosurgery, the People’s Hospital of Jilin Province, Changchun 130021, P. R. China
  • Received:2008-12-01 Revised:2009-02-02 Online:2009-11-25 Published:2010-01-25
  • Contact: RUO Qi. E-mail: liluoqi@163.com

PDF (PC)

Abstract:

The adenovirus E1A proteins are primarily concerned with modulating cellular metabolism to make the cell more susceptible to virus replication. In this study, adenovirus E1A gene was amplified via PCR on the HEK-293 cells genome and cloned into prokaryotic expression vector pRsetB. The recombinant plasmid pRsetB-E1A was expressed in E. coli BL21 and the relative molecular mass(Mr) of expressed fusion protein was approximately 36000. The recombinant protein was purified on a Ni-NIA agarose column and detected by SDS-PAGE and Western blot. The purified recombinant protein was then injected into rabbits and anti-E1A polyclonal antibody with high titer was obtained.

Key words: Adenovirus; E1A; Prokaryotic expression; Polyclonal antibody