Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

doi:10.1007/s40242-023-3046-9

高等学校化学研究 ›› 2023, Vol. 39 ›› Issue (3): 472-480.doi: 10.1007/s40242-023-3046-9

Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

Hao Wang¹, Yue Zhang³, Liuqing Zhao², Zeying He¹, Jingran Zhang², Bingjie Liu², Xiaowei Liu¹, Yanwei Zhang¹

1. Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, P. R. China;
2. Shanghai SCIEX Analytical Instrument Trading Co., Ltd., Shanghai, 200335, P. R. China;
3. Department of Applied Chemistry, China Agricultural University, Beijing, 100193, P. R. China

收稿日期:2023-02-27 发布日期:2023-05-25
通讯作者: Yue Zhang, Yanwei Zhang E-mail:17854166935@163.com;zhangyanwei@caas.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (No.41807391) and the Tianjin Natural Science Foundation, China (No.18JCQNJC09100).

Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

WANG Hao, ZHANG Yue, ZHAO Liuqing, HE Zeying, ZHANG Jingran, LIU Bingjie, LIU Xiaowei, ZHANG Yanwei

1. Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, P. R. China;
2. Shanghai SCIEX Analytical Instrument Trading Co., Ltd., Shanghai, 200335, P. R. China;
3. Department of Applied Chemistry, China Agricultural University, Beijing, 100193, P. R. China

Received:2023-02-27 Published:2023-05-25
Contact: Yue Zhang, Yanwei Zhang E-mail:17854166935@163.com;zhangyanwei@caas.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (No.41807391) and the Tianjin Natural Science Foundation, China (No.18JCQNJC09100).

摘要/Abstract

摘要： The ecotoxicity of perfluorooctane sulfonate(PFOS) to plants has been reported, but the lack of study on the effect of PFOS isomers is of concern. In this paper, the effect mechanism of Arabidopsis thaliana(A. thaliana) against two different concentrations of L-PFOS or perfluoro-3-methylheptanesulfonate (P3MHpS) was investigated via metabolomics. The metabolism of Arabidopsis thaliana leaves was investigated via the metabolomics strategy and statistical analysis. And 41 biomarkers for L-PFOS and 32 biomarkers for P3MHpS were found and identified. There was no significant difference of saccharides between L-PFOS and P3MHpS. More amino acids were affected by P3MHpS than by L-PFOS in high-level exposure group, while the opposite phenomenon was observed in low-level exposure group. More phytohormones were affected by L-PFOS than by P3MHpS in the high-level exposure group, however, the elevated level of indole phytohormones was significantly higher by P3MHpS than by L-PFOS. The level of up-regulation of (poly)phenols in L-PFOS group was significantly higher than that in P3MHpS group. The affected aliphatic glucosinolate metabolism was firstly observed with higher up-regulation induced by L-PFOS than by P3MHpS. New insight of phytotoxicity of PFOS isomers to plants was provided.

关键词: L-Perfluorooctane sulfonate(L-PFOS), Perfluoro-3-methylheptanesulfonate(P3MHpS), Phytotoxicity, Metabolomics, Glucosinolate

Abstract: The ecotoxicity of perfluorooctane sulfonate(PFOS) to plants has been reported, but the lack of study on the effect of PFOS isomers is of concern. In this paper, the effect mechanism of Arabidopsis thaliana(A. thaliana) against two different concentrations of L-PFOS or perfluoro-3-methylheptanesulfonate (P3MHpS) was investigated via metabolomics. The metabolism of Arabidopsis thaliana leaves was investigated via the metabolomics strategy and statistical analysis. And 41 biomarkers for L-PFOS and 32 biomarkers for P3MHpS were found and identified. There was no significant difference of saccharides between L-PFOS and P3MHpS. More amino acids were affected by P3MHpS than by L-PFOS in high-level exposure group, while the opposite phenomenon was observed in low-level exposure group. More phytohormones were affected by L-PFOS than by P3MHpS in the high-level exposure group, however, the elevated level of indole phytohormones was significantly higher by P3MHpS than by L-PFOS. The level of up-regulation of (poly)phenols in L-PFOS group was significantly higher than that in P3MHpS group. The affected aliphatic glucosinolate metabolism was firstly observed with higher up-regulation induced by L-PFOS than by P3MHpS. New insight of phytotoxicity of PFOS isomers to plants was provided.

Key words: L-Perfluorooctane sulfonate(L-PFOS), Perfluoro-3-methylheptanesulfonate(P3MHpS), Phytotoxicity, Metabolomics, Glucosinolate

Hao Wang, Yue Zhang, Liuqing Zhao, Zeying He, Jingran Zhang, Bingjie Liu, Xiaowei Liu, Yanwei Zhang. Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics[J]. 高等学校化学研究, 2023, 39(3): 472-480.

WANG Hao, ZHANG Yue, ZHAO Liuqing, HE Zeying, ZHANG Jingran, LIU Bingjie, LIU Xiaowei, ZHANG Yanwei. Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics[J]. Chemical Research in Chinese Universities, 2023, 39(3): 472-480.

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Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

Effect Mechanism of Low-level PFOS Isomers to A. thaliana Leaves Using High-throughput Untargeted Metabolomics

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