Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery

doi:10.1007/s40242-021-1084-8

高等学校化学研究 ›› 2021, Vol. 37 ›› Issue (3): 488-495.doi: 10.1007/s40242-021-1084-8

Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery

HONG Huoyan, ZHANG Dongdong, LIN Si, HAN Feng, WANG Kaili, JIANG Di, WU Jinglei, MO Xiumei, WANG Hongsheng

Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China

收稿日期:2021-03-03 修回日期:2021-04-12 出版日期:2021-06-01 发布日期:2021-04-12
通讯作者: WANG Hongsheng, WU Jinglei E-mail:whs@dhu.edu.cn;jw@dhu.edu.cn
基金资助:
This work was supported by the Project of the Science & Technology Commission of Shanghai Municipality, China(Nos.18490740400, 20DZ2254900).

Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery

HONG Huoyan, ZHANG Dongdong, LIN Si, HAN Feng, WANG Kaili, JIANG Di, WU Jinglei, MO Xiumei, WANG Hongsheng

Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China

Received:2021-03-03 Revised:2021-04-12 Online:2021-06-01 Published:2021-04-12
Contact: WANG Hongsheng, WU Jinglei E-mail:whs@dhu.edu.cn;jw@dhu.edu.cn
Supported by:
This work was supported by the Project of the Science & Technology Commission of Shanghai Municipality, China(Nos.18490740400, 20DZ2254900).

摘要/Abstract

摘要： Transdermal drug delivery system(TDDS) facilitates the controlled release of active ingredients penetrating through the skin, avoiding the liver first pass effect. Electrospinning is a simple process to fabricate ultrafine fibers with a higher specific surface area, making them excellent candidates for drug delivery. In current work, a novel silk fibroin(SF) nanofiber loaded with cationic ethosomes(CEs) was prepared via green electrospinning. The data of Fourier transform infrared spectroscopy(FTIR) and laser scanning confocal microscopy(LSCM) confirmed the existence of CEs in the SF nanofibers. The morphology of the nanofibers was not significantly affected by the incorporation of CEs as shown by scanning electron microscopy(SEM) images. The CEs-loaded SF nanofibrous patch (CEs-SFnP) showed good cytocompatibility as proved by both cell counting Kit-8(CCK-8) assay and SEM. Using doxorubicin hydrochloride(Dox) as a model drug, the transdermal performance of CEs-SFnP was evaluated through Franz diffusion cell against mouse skin. The results indicated that CEs-SFnP can effectively deliver drug into the skin, with a much higher permeation rate than the normal nanofibers without CEs. The as-spun CEs-SFnP in this study could find promising applications in TDDS.

关键词: Transdermal drug delivery, Silk fibroin, Ethosome, Green electrospinning

Abstract: Transdermal drug delivery system(TDDS) facilitates the controlled release of active ingredients penetrating through the skin, avoiding the liver first pass effect. Electrospinning is a simple process to fabricate ultrafine fibers with a higher specific surface area, making them excellent candidates for drug delivery. In current work, a novel silk fibroin(SF) nanofiber loaded with cationic ethosomes(CEs) was prepared via green electrospinning. The data of Fourier transform infrared spectroscopy(FTIR) and laser scanning confocal microscopy(LSCM) confirmed the existence of CEs in the SF nanofibers. The morphology of the nanofibers was not significantly affected by the incorporation of CEs as shown by scanning electron microscopy(SEM) images. The CEs-loaded SF nanofibrous patch (CEs-SFnP) showed good cytocompatibility as proved by both cell counting Kit-8(CCK-8) assay and SEM. Using doxorubicin hydrochloride(Dox) as a model drug, the transdermal performance of CEs-SFnP was evaluated through Franz diffusion cell against mouse skin. The results indicated that CEs-SFnP can effectively deliver drug into the skin, with a much higher permeation rate than the normal nanofibers without CEs. The as-spun CEs-SFnP in this study could find promising applications in TDDS.

Key words: Transdermal drug delivery, Silk fibroin, Ethosome, Green electrospinning

HONG Huoyan, ZHANG Dongdong, LIN Si, HAN Feng, WANG Kaili, JIANG Di, WU Jinglei, MO Xiumei, WANG Hongsheng. Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery[J]. 高等学校化学研究, 2021, 37(3): 488-495.

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Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery

Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery

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