Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film

高等学校化学研究 ›› 2009, Vol. 25 ›› Issue (4): 550-555.

Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film

ZHENG Hai-tao^1*, DONG Li-ping¹, LIU Hai-hui¹, JIA Hai-lian¹ and SUYE Shin-ichiro²

1. School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, P. R. China;
2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan

收稿日期:2008-02-28 修回日期:2008-06-16 出版日期:2009-07-25 发布日期:2009-10-16
通讯作者: ZHENG Hai-tao. E-mail: zhenght999@hotmail.com
基金资助:
Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education, China and the Natural Science Foundation of Tianjin City, China(No.06YFJMJC15000).

Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film

ZHENG Hai-tao^1*, DONG Li-ping¹, LIU Hai-hui¹, JIA Hai-lian¹ and SUYE Shin-ichiro²

1. School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, P. R. China;
2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan

Received:2008-02-28 Revised:2008-06-16 Online:2009-07-25 Published:2009-10-16
Contact: ZHENG Hai-tao. E-mail: zhenght999@hotmail.com
Supported by:
Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education, China and the Natural Science Foundation of Tianjin City, China(No.06YFJMJC15000).

摘要/Abstract

Abstract:

Multilayer film was fabricated on an electrode surface by alternate layer-by-layer(LBL) adsorption of polycationic redox polymer(PEI-Fc) and dye-linked L-proline dehydrogenase(L-proDH). The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry, and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline. It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc. A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode, and the calculated currents fitted well with the experimental data. The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer.

Key words: Electron communication; Dye-linked L-proline dehydrogenase; Layer-by-layer adsorption; Electrode kinetic

ZHENG Hai-tao*, DONG Li-ping, LIU Hai-hui, JIA Hai-lian and SUYE Shin-ichiro. Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film[J]. 高等学校化学研究, 2009, 25(4): 550-555.

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Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film

Electron Communication Between Electrode and Dye-Linked L-Proline Dehydrogenase in Organized Layer-by-Layer Multilayer Film

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