Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route

doi:10.1007/s40242-019-8371-7

高等学校化学研究 ›› 2019, Vol. 35 ›› Issue (5): 901-907.doi: 10.1007/s40242-019-8371-7

Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route

LIU Zhenlei, JIA Qingyang, ZHAO Xiufen, LI Shuping, SHEN Jian, LI Xiaodong

Jiangsu Key Laboratory of Biofunctional Material, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210023, P. R. China

收稿日期:2018-11-29 出版日期:2019-10-01 发布日期:2019-09-24
通讯作者: LI Shuping, SHEN Jian E-mail:lishuping@njnu.edu.cn;shenjian@njnu.edu.cn
基金资助:
Supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials.

Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route

LIU Zhenlei, JIA Qingyang, ZHAO Xiufen, LI Shuping, SHEN Jian, LI Xiaodong

Jiangsu Key Laboratory of Biofunctional Material, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210023, P. R. China

Received:2018-11-29 Online:2019-10-01 Published:2019-09-24
Contact: LI Shuping, SHEN Jian E-mail:lishuping@njnu.edu.cn;shenjian@njnu.edu.cn
Supported by:
Supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials.

摘要/Abstract

摘要： The intercalation of methotrexate(MTX) into layered double hydroxides(LDHs) via an exfoliation-reassembly process was performed and the resulting hybrids were then characterized by X-ray diffractiometry(XRD), Fourier transform infrared(FTIR) spectroscopy, thermogravimetry(TG) and differential scanning calorimetry(DSC), etc. In the synthesis procedure, LDHs particles were firstly delaminated, and the reassembly process was performed to get MTX intercalated LDH(MTX/LDHs) hybrids. The reassembly conditions, such as reassembly time and different reassembly solvent, were studied emphatically. It was found that 20 min is the optimum time and formamide is the most suitable solvent in the reassembly process. At last, the controlled release property and the drug-loading capacity were examined emphatically to reveal that MTX/LDHs hybrids reassembled in formamide exhibit the highest drug-loading capacity. The cytotoxicity of the hybrids was estimated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,thiazolyl blue tetrazolium bromide(MTT) assays on the human lung cancer cells(A549), with the results showing that MTX/LDHs hybrids have effective suppress role on the proliferation of cancer cells, and the hybrids obtained from the formamide solution present much better anticancer effect than the other samples.

关键词: Methotrexatum, Layered double hydroxide, Exfoliation-reassembly process

Abstract: The intercalation of methotrexate(MTX) into layered double hydroxides(LDHs) via an exfoliation-reassembly process was performed and the resulting hybrids were then characterized by X-ray diffractiometry(XRD), Fourier transform infrared(FTIR) spectroscopy, thermogravimetry(TG) and differential scanning calorimetry(DSC), etc. In the synthesis procedure, LDHs particles were firstly delaminated, and the reassembly process was performed to get MTX intercalated LDH(MTX/LDHs) hybrids. The reassembly conditions, such as reassembly time and different reassembly solvent, were studied emphatically. It was found that 20 min is the optimum time and formamide is the most suitable solvent in the reassembly process. At last, the controlled release property and the drug-loading capacity were examined emphatically to reveal that MTX/LDHs hybrids reassembled in formamide exhibit the highest drug-loading capacity. The cytotoxicity of the hybrids was estimated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,thiazolyl blue tetrazolium bromide(MTT) assays on the human lung cancer cells(A549), with the results showing that MTX/LDHs hybrids have effective suppress role on the proliferation of cancer cells, and the hybrids obtained from the formamide solution present much better anticancer effect than the other samples.

Key words: Methotrexatum, Layered double hydroxide, Exfoliation-reassembly process

LIU Zhenlei, JIA Qingyang, ZHAO Xiufen, LI Shuping, SHEN Jian, LI Xiaodong. Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route[J]. 高等学校化学研究, 2019, 35(5): 901-907.

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Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route

Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route

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