Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

doi:10.1007/s40242-019-9101-x

高等学校化学研究 ›› 2019, Vol. 35 ›› Issue (4): 686-692.doi: 10.1007/s40242-019-9101-x

Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

DUAN Mengna¹, WU Xiaofeng², YUAN Long³, ZHANG Zhiying¹, ZHANG Yan¹, ZHOU Yanmin¹

1. Hospital of Stomatology, Jilin University, Changchun 130021, P. R. China;
2. State Key Laboratory of Inorganic Synthesis & amp;Preparative Chemistry, Jilin University, Changchun 130012, P. R. China;
3. Key Laboratory of Functional Materials Physics and Chemistry, Ministry of Education, Jilin Normal University, Changchun 130103, P. R. China

收稿日期:2019-04-10 修回日期:2019-05-05 出版日期:2019-08-01 发布日期:2019-08-01
通讯作者: ZHOU Yanmin E-mail:zhouym62@126.com
基金资助:
Supported by the Jilin Provincial Health and Family Planning Young Science and Technology Backbone Training Program, China(No.2016Q022) and the Jilin Provincial Department of Education Science and Technology Program, China (No.JJKH20190104KJ).

Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

DUAN Mengna¹, WU Xiaofeng², YUAN Long³, ZHANG Zhiying¹, ZHANG Yan¹, ZHOU Yanmin¹

1. Hospital of Stomatology, Jilin University, Changchun 130021, P. R. China;
2. State Key Laboratory of Inorganic Synthesis & amp;Preparative Chemistry, Jilin University, Changchun 130012, P. R. China;
3. Key Laboratory of Functional Materials Physics and Chemistry, Ministry of Education, Jilin Normal University, Changchun 130103, P. R. China

Received:2019-04-10 Revised:2019-05-05 Online:2019-08-01 Published:2019-08-01
Contact: ZHOU Yanmin E-mail:zhouym62@126.com
Supported by:
Supported by the Jilin Provincial Health and Family Planning Young Science and Technology Backbone Training Program, China(No.2016Q022) and the Jilin Provincial Department of Education Science and Technology Program, China (No.JJKH20190104KJ).

摘要/Abstract

摘要： Compared with the traditional dental implant, TixOs^® manufactured by direct laser metal forming(DLMF) technology exhibits improved capability for bone osteointegration due to its porous surface structure, and has achieved remarkable clinical effect. However, like the traditional titanium and other alloyed implants, the porous titanium implant TixOs^® also has relatively weak bioactivity. To address this issue, a proper surface modification method may be needed. Hydroxyapatite(HA) has been widely used in implant surface coating for its similar chemical composition to bone tissue and its osteoconductive properties. Thus, combining TixOs^® implants with hydroxyapatite can be an efficient way to enhance their bioactivity. We herewith reported a competent pulsed laser deposition(PLD) method of coating nano-sized HA thin film onto the porous TixOs^® implant. The HA coatings were characterized by means of scanning electron microscopy(SEM), energy dispersive X-ray spectroscopy(EDS), X-ray photoelectron spectroscopy(XPS) and focused ion beam(FIB) method, and nanocrystal sized thin HA films were identified on the surface of TixOs^® implants. The low cytotoxicity and improved cell proliferation ability of HA coated implants were further tested and verified using MC 3T3 E1 cells with the consideration of the controlling group. Our results show that a stable and bioactive HA thin film is able to form on the surface of the porous titanium implant by PLD method. This may benefit the further clinical application of TixOs^® implants.

关键词: Hydroxyapatite, Pulsed laser deposition, Porous titanium, Bioactivity

Abstract: Compared with the traditional dental implant, TixOs^® manufactured by direct laser metal forming(DLMF) technology exhibits improved capability for bone osteointegration due to its porous surface structure, and has achieved remarkable clinical effect. However, like the traditional titanium and other alloyed implants, the porous titanium implant TixOs^® also has relatively weak bioactivity. To address this issue, a proper surface modification method may be needed. Hydroxyapatite(HA) has been widely used in implant surface coating for its similar chemical composition to bone tissue and its osteoconductive properties. Thus, combining TixOs^® implants with hydroxyapatite can be an efficient way to enhance their bioactivity. We herewith reported a competent pulsed laser deposition(PLD) method of coating nano-sized HA thin film onto the porous TixOs^® implant. The HA coatings were characterized by means of scanning electron microscopy(SEM), energy dispersive X-ray spectroscopy(EDS), X-ray photoelectron spectroscopy(XPS) and focused ion beam(FIB) method, and nanocrystal sized thin HA films were identified on the surface of TixOs^® implants. The low cytotoxicity and improved cell proliferation ability of HA coated implants were further tested and verified using MC 3T3 E1 cells with the consideration of the controlling group. Our results show that a stable and bioactive HA thin film is able to form on the surface of the porous titanium implant by PLD method. This may benefit the further clinical application of TixOs^® implants.

Key words: Hydroxyapatite, Pulsed laser deposition, Porous titanium, Bioactivity

DUAN Mengna, WU Xiaofeng, YUAN Long, ZHANG Zhiying, ZHANG Yan, ZHOU Yanmin. Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant[J]. 高等学校化学研究, 2019, 35(4): 686-692.

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Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

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