Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents

doi:10.1007/s40242-017-7048-3

高等学校化学研究 ›› 2017, Vol. 33 ›› Issue (6): 903-907.doi: 10.1007/s40242-017-7048-3

Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents

WANG Zhiyuan, LUO Wen, FU Junying, LI Zhibing, WANG Zhongming, LÜ Pengmei

Key Laboratory of Renewable Energy of Chinese Academy of Sciences, Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, P. R. China

收稿日期:2017-02-13 修回日期:2017-04-06 出版日期:2017-12-01 发布日期:2017-09-26
通讯作者: LÜ Pengmei,E-mail:lvpm@ms.giec.ac.cn E-mail:lvpm@ms.giec.ac.cn
基金资助:
Supported by the Special Funds of Applied Science and Technology Research of Guangdong Province, China(No. 2015B020241002) and the Scientific and Technological Project of Guangdong Province, China(No. 2016A010104008).

Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents

WANG Zhiyuan, LUO Wen, FU Junying, LI Zhibing, WANG Zhongming, LÜ Pengmei

Key Laboratory of Renewable Energy of Chinese Academy of Sciences, Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, P. R. China

Received:2017-02-13 Revised:2017-04-06 Online:2017-12-01 Published:2017-09-26
Contact: LÜ Pengmei,E-mail:lvpm@ms.giec.ac.cn E-mail:lvpm@ms.giec.ac.cn
Supported by:
Supported by the Special Funds of Applied Science and Technology Research of Guangdong Province, China(No. 2015B020241002) and the Scientific and Technological Project of Guangdong Province, China(No. 2016A010104008).

摘要/Abstract

摘要： Kojic dipalmitate(KDP) was synthesized by the way of esterification of palmitic and kojic acids in organic solvent, with double-lipase as the biocatalyst. Four commercially available lipases(Amano PS, Novozym 435, Lipozyme TL IM and Lipozyme RM IM) were used to group six double-lipase combinations. These combinations were studied and Amano PS-Novozym 435 was found to have the best efficiency and was selected for optimizing the reaction conditions. The optimal reaction conditions were as follows. The mass ratio of Amano PS to Novozyme 435 was 1:1.5, with stirring speed of 500 r/min, substrates molar ratio of 1:2, 50℃, 5%(mass fraction) catalyst dosage of kojic acid quantity and using acetone as co-solvent. Under these conditions, the diesterification of C5 and C7 hydroxyl groups of kojic acid molecule could be well catalyzed by double-lipase and realize a high KDP yield of 85%.

关键词: Kojic acid, Palmitic acid, Kojic dipalmitate, Double-lipase catalysis, Diesterification

Abstract: Kojic dipalmitate(KDP) was synthesized by the way of esterification of palmitic and kojic acids in organic solvent, with double-lipase as the biocatalyst. Four commercially available lipases(Amano PS, Novozym 435, Lipozyme TL IM and Lipozyme RM IM) were used to group six double-lipase combinations. These combinations were studied and Amano PS-Novozym 435 was found to have the best efficiency and was selected for optimizing the reaction conditions. The optimal reaction conditions were as follows. The mass ratio of Amano PS to Novozyme 435 was 1:1.5, with stirring speed of 500 r/min, substrates molar ratio of 1:2, 50℃, 5%(mass fraction) catalyst dosage of kojic acid quantity and using acetone as co-solvent. Under these conditions, the diesterification of C5 and C7 hydroxyl groups of kojic acid molecule could be well catalyzed by double-lipase and realize a high KDP yield of 85%.

Key words: Kojic acid, Palmitic acid, Kojic dipalmitate, Double-lipase catalysis, Diesterification

WANG Zhiyuan, LUO Wen, FU Junying, LI Zhibing, WANG Zhongming, LÜ Pengmei. Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents[J]. 高等学校化学研究, 2017, 33(6): 903-907.

WANG Zhiyuan, LUO Wen, FU Junying, LI Zhibing, WANG Zhongming, LÜ Pengmei. Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents[J]. Chemical Research in Chinese Universities, 2017, 33(6): 903-907.

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Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents

Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents

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