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高等学校化学研究 ›› 2016, Vol. 32 ›› Issue (3): 402-405.doi: 10.1007/s40242-016-5519-6

• Articles • 上一篇    下一篇

Proteomics Analysis of Up-regulated NPM1 Protein in Colorectal Cancer

WANG Hai1, HE Chengyan1, YANG Zhaowei1, GAO Shen1, LI Lingxia1, SUN Xiaoying1, FANG Ling1, LIU Ning2, LI Hongjun1   

  1. 1. Jilin University China-Japan Union Hospital, Changchun 130033, P. R. China;
    2. The Second Hospital of Jilin University, Changchun 130041, P. R. China
  • 收稿日期:2015-12-31 修回日期:2016-03-02 出版日期:2016-06-01 发布日期:2016-04-05
  • 通讯作者: LIU Ning, LI Hongjun E-mail:liu_ning@jlu.edu.cn;lihongjun1960@126.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(Nos.81572082, 81472030, 20973074), the Science Foundation of Jilin Provincial Science & Technology Department, China(No.2011713, 20130413017GH, 20150414015GH), the Health and Family Planning Commission of Jilin Province, China(No.2015Z041) and the Scientific Support Project from Bethune Department of Jilin University, China(Nos.2012210, 2013207051).

Proteomics Analysis of Up-regulated NPM1 Protein in Colorectal Cancer

WANG Hai1, HE Chengyan1, YANG Zhaowei1, GAO Shen1, LI Lingxia1, SUN Xiaoying1, FANG Ling1, LIU Ning2, LI Hongjun1   

  1. 1. Jilin University China-Japan Union Hospital, Changchun 130033, P. R. China;
    2. The Second Hospital of Jilin University, Changchun 130041, P. R. China
  • Received:2015-12-31 Revised:2016-03-02 Online:2016-06-01 Published:2016-04-05
  • Contact: LIU Ning, LI Hongjun E-mail:liu_ning@jlu.edu.cn;lihongjun1960@126.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.81572082, 81472030, 20973074), the Science Foundation of Jilin Provincial Science & Technology Department, China(No.2011713, 20130413017GH, 20150414015GH), the Health and Family Planning Commission of Jilin Province, China(No.2015Z041) and the Scientific Support Project from Bethune Department of Jilin University, China(Nos.2012210, 2013207051).

被引次数

1

摘要：

In order to identify potential protein targets involved in colorectal cancer(CRC), we used a liquid chromatography coupled with mass spectrometry(LC-MS)/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin 1(NPM1) was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.

关键词: Liquid chromatography-mass spectrometry(LC-MS), Colorectal cancer(CRC), Nucleophosmin 1(NPM1)

Abstract:

In order to identify potential protein targets involved in colorectal cancer(CRC), we used a liquid chromatography coupled with mass spectrometry(LC-MS)/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin 1(NPM1) was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.

Key words: Liquid chromatography-mass spectrometry(LC-MS), Colorectal cancer(CRC), Nucleophosmin 1(NPM1)