Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

doi:10.1007/s40242-015-5207-y

高等学校化学研究 ›› 2015, Vol. 31 ›› Issue (6): 914-918.doi: 10.1007/s40242-015-5207-y

Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

MA Yinghui¹, LIU Yuanyuan¹, WEI Mengying¹, SONG Fengrui², LIU Zhongying¹, PI Zifeng²

1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

收稿日期:2015-05-25 修回日期:2015-07-06 出版日期:2015-11-01 发布日期:2015-11-24
通讯作者: LIU Zhongying, PI Zifeng E-mail:liuzy@jlu.edu.cn;mslab21@ciac.ac.cn
基金资助:
Supported by the National Natural Science Foundation of China(Nos.81073040, 81274046, 81173507), the Jilin Provincial Science and Technology Agency-funded Projects, China(No.20150414040GH) and the National Basic Research Program of China(Nos.2011CB505300, 2011CB505305).

Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

MA Yinghui¹, LIU Yuanyuan¹, WEI Mengying¹, SONG Fengrui², LIU Zhongying¹, PI Zifeng²

1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2015-05-25 Revised:2015-07-06 Online:2015-11-01 Published:2015-11-24
Contact: LIU Zhongying, PI Zifeng E-mail:liuzy@jlu.edu.cn;mslab21@ciac.ac.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.81073040, 81274046, 81173507), the Jilin Provincial Science and Technology Agency-funded Projects, China(No.20150414040GH) and the National Basic Research Program of China(Nos.2011CB505300, 2011CB505305).

摘要/Abstract

摘要：

The Caco-2 cells have been recognized as effective tools to be applied to imitating the drug absorption in human intestine for the transport of drug. Herein, Caco-2 cell monolayer model was used to study the transport of the ginsenoside compatibility with Veratrum nigrum in different proportions. A specific high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS) method was developed for the semiquantitative determination of ginsenoside in intestinal transport with Dioscin as an internal standard. For the Caco-2 model constructed, two influencing factors were investigated, including time and concentration. The results suggest that the absorption of ginsenoside Re, Rg1, Rb1, Rc, Rb2 and Rd are time- and concentration-dependent and the excretions of Rb1, Rc, Rb2 and Rd have a relatronship with some transport proteins. The bioavailability of the ginsenosides has reduced compared to the single Panax ginseng extract when compatibility with a certain amount of Veratrum nigrum.

关键词: High performance liquid chromatography-electrospray ionization-mass spectroscopy(HPLC-ESI-MS), Panax ginseng, Ginsenoside, Veratrum nigrum, Transport, Caco-2 cell monolayer model

Abstract:

Key words: High performance liquid chromatography-electrospray ionization-mass spectroscopy(HPLC-ESI-MS), Panax ginseng, Ginsenoside, Veratrum nigrum, Transport, Caco-2 cell monolayer model

MA Yinghui, LIU Yuanyuan, WEI Mengying, SONG Fengrui, LIU Zhongying, PI Zifeng. Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method[J]. 高等学校化学研究, 2015, 31(6): 914-918.

参考文献

[1] Ru W. W., Wang D. L., Xu Y. P., He X. X., Sun Y. E., Qian L. Y., Zhou X. S., Qin Y. F., Drug Discoveries and Therapeutics, 2015, 9, 23
[2] Park J., Jeon Y. D., Kim H. L., Lim H., Jung Y., Youn D. H., Jeong M. Y., Kim H. J., Kim S. H., Kim S. J., Hong S. H., Um J. Y., Evidence-based Complementary and Alternative Medicine, 2013, 10, 1155
[3] Zhang X., Song F. R., Wang L. S., Liu Z. Q., Liu S. Y., Acta Chim. Sinica, 2007, 65, 829
[4] Lu Y. L., Sun A. H., Gao Y., Jiang Y., Mil. Med. Sci., 2014, 38, 285
[5] Wang Y. G., Gao Y., Chai B. X., Chen P., Tan H. L., Zhao Y. H., Xiao C. R., Sun Y. Y., Zhu L. J., Pharm. J. Chin. PLA, 2014, 29, 366
[6] Wang Y. G., Gao Y., Ma Z. C., Tan H. L., Xiao C. R., Zhao Y. H., Chin. J. Pharmacol. Toxicol., 2013, 27, 982
[7] Lin Y., Shang E. X., Xu Y., Yang Y., Song F. R., Liu Z. Q., Chinese Journal of Experimental Traditional Medical Formulae, 2014, 20, 124
[8] Marco A., Reis J., Ana C. M., Eudes, Silva V., Teresa D. C., Frank P. A., Whocely V. C., Biomedical Chromatography, 2014, 8, 26
[9] Sun S., Zhang H., Sun F. F., Zhong Y. Q., Chai Y. F., Zhang G. Q., Biomedical Chromatography, 2014, 5, 25
[10] Akhir N. M., Chua L. S., Majid F. A. A., Sarmidi M. R., Br. J. Med. Med. Res., 2011, 1, 397
[11] Artursson P., J. Pharm. Sci., 1990, 6, 476
[12] Moradi S. V., Varamini P., Toth I., J. Pharm. Sci., 2014, 103, 3217
[13] Liang Y., Zhou Y., Zhang J., Rao T., Zhou L., Xing R., Wang Q., Fu H., Hao K., Xie L., Wang G., PLoS One, 2014, 9, e98717
[14] Ma J. W., Kan H., Ma Y. H., Men L. H., Pi Z. F., Liu Z. Q., Liu Z. Y., Chem. Res. Chinese Universities, 2014, 30(3), 731
[15] Liu G. L., Jilin Agriculture, 2011, 3, 290
[16] Yang Y., Yang L. Y., Dai J. F., Lishizhen Medicine and Materia Medica Research, 2012, 23, 3121
[17] Yang Q. Y., Li X. Y., Liu G. L., Chinese Pharmaceutical Journal, 2013, 48, 1233
[18] Sun G. Z., Wang J. Y., Liu Z., Wang L. J., Li X. G., Journal of Jilin Agricultural University, 2005, 27, 299

Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method

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