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高等学校化学研究 ›› 2013, Vol. 29 ›› Issue (4): 638-641.doi: 10.1007/s40242-013-3012-z

• Articles • 上一篇    下一篇

Purification and Characterization of a Novel Tetradecapeptide from Ginseng Polypeptides with Enhancing Memory Activity for Mice

LUO Hao-ming1, JIANG Rui-zhi2, YANG Xiao-hong1, CHEN Ying-hong2, HONG Tie1, WANG Ying2   

  1. 1. School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P. R. China;
    2. Jilin Academy of Chinese Medicine and Material Medical Science, Changchun 130012, P. R. China
  • 收稿日期:2013-01-08 修回日期:2013-03-01 出版日期:2013-08-01 发布日期:2013-07-15
  • 通讯作者: WANG Ying E-mail:wangying02231@sina.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.81173532), the Science and Technology Development Project of Jilin Province, China(No.201201086), the Traditional Chinese Medicine Science and Technology Project from Jilin Provincial Administration of Traditional Chinese Medicine, China(No.2012-114).

Purification and Characterization of a Novel Tetradecapeptide from Ginseng Polypeptides with Enhancing Memory Activity for Mice

LUO Hao-ming1, JIANG Rui-zhi2, YANG Xiao-hong1, CHEN Ying-hong2, HONG Tie1, WANG Ying2   

  1. 1. School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P. R. China;
    2. Jilin Academy of Chinese Medicine and Material Medical Science, Changchun 130012, P. R. China
  • Received:2013-01-08 Revised:2013-03-01 Online:2013-08-01 Published:2013-07-15
  • Contact: WANG Ying E-mail:wangying02231@sina.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.81173532), the Science and Technology Development Project of Jilin Province, China(No.201201086), the Traditional Chinese Medicine Science and Technology Project from Jilin Provincial Administration of Traditional Chinese Medicine, China(No.2012-114).

被引次数

6

摘要：

Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaccharides and polypeptides, a DEAE-Sephadex A-50 colum was used. The enhanced memory activity in mice was studied by Mirros water maze tesk in mice. The dose of oligosacchrides, polypeptides or Piracetam was 30 mg/kg per day with intraperitoneal administration. The oligosaccharides did not show enhancing memory effect, but polypeptides did show. This result demonstrates that the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory effect was polypeptides. The purification of the polypeptides was performed on a Sephadex G-25 column. A novel tetradecapeptide was purified from the polypeptides and its structure was determined by liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry(LC-ESI-Q-TOF-MS) with the amino acid sequence of Lys-Ser-Leu-Thr-Leu-Thr-Ser-Ser-Leu-Ser-Tyr-Thr-Asp-Ser.

关键词: Panax ginseng, Polypeptide, Tetradecapeptide, Enhanced memory activity, Liquid chromatography-tandem mass spectrometry(LC-MS/MS)

Abstract:

Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaccharides and polypeptides, a DEAE-Sephadex A-50 colum was used. The enhanced memory activity in mice was studied by Mirros water maze tesk in mice. The dose of oligosacchrides, polypeptides or Piracetam was 30 mg/kg per day with intraperitoneal administration. The oligosaccharides did not show enhancing memory effect, but polypeptides did show. This result demonstrates that the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory effect was polypeptides. The purification of the polypeptides was performed on a Sephadex G-25 column. A novel tetradecapeptide was purified from the polypeptides and its structure was determined by liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry(LC-ESI-Q-TOF-MS) with the amino acid sequence of Lys-Ser-Leu-Thr-Leu-Thr-Ser-Ser-Leu-Ser-Tyr-Thr-Asp-Ser.

Key words: Panax ginseng, Polypeptide, Tetradecapeptide, Enhanced memory activity, Liquid chromatography-tandem mass spectrometry(LC-MS/MS)