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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (2): 259-263 .

• Articles • 上一篇    下一篇

Establishment of a Tumor-bearing Mouse Model Stably Expressing Human Tumor Antigens Survivin and MUC1 VNTRs

ZHANG Li-xing1,2, DU Jian-shi1, WANG Yu-qian2, LIU Chen-lu2, XIA Qiu2, ZHANG Xi-zhen2, CONG Xian-ling1, ZHANG Hai-hong2   

  1. 1. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. National Engineering Lab of AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, P. R. China
  • 收稿日期:2011-10-14 修回日期:2011-11-30 出版日期:2012-03-25 发布日期:2012-03-08
  • 通讯作者: CONG Xian-ling, ZHANG Hai-hong E-mail:congxl888@hotmail.com; zhanghh@jlu.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.30872396), the Scientific Research Foundation of Jilin Province, China(Nos.20080709, 200905169) and the Jilin University Basic Research Project, China(No.200903255).

Establishment of a Tumor-bearing Mouse Model Stably Expressing Human Tumor Antigens Survivin and MUC1 VNTRs

ZHANG Li-xing1,2, DU Jian-shi1, WANG Yu-qian2, LIU Chen-lu2, XIA Qiu2, ZHANG Xi-zhen2, CONG Xian-ling1, ZHANG Hai-hong2   

  1. 1. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. National Engineering Lab of AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, P. R. China
  • Received:2011-10-14 Revised:2011-11-30 Online:2012-03-25 Published:2012-03-08
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30872396), the Scientific Research Foundation of Jilin Province, China(Nos.20080709, 200905169) and the Jilin University Basic Research Project, China(No.200903255).

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被引次数

6

摘要： The eukaryotic vectors VR1012 expressing survivin or 33 tandem repeats of human mucin 1(MUC1) (VNTRs), namely, VR1012-S and VR1012-VNTR(VNTR=variable number of tandem repeat), were constructed by cloning survivin and VNTR genes into VR1012, respectively. The eukaryotic vector pEGFP expressing survivin and MUC1 VNTRs fusion gene pEGFP-MS was also constructed. Mouse melanoma cell line(B16) stably expressing survivin and MUC1 VNTRs(MS+B16) was established by Lipofectamine-mediated transfection of pEGFP-MS into B16 cells. EGFP expression in MS+B16 cells was observed using a fluorescent microscope and survivin and MUC1 VNTRs(MS) expression was confirmed by means of Western blot analysis. A syngenic graft tumor model was gene- rated by subcutaneous injection of MS+B16 cells into C57/BL6 mice and tumor size increased rapidly with time in a cell number dependent manner. After the third immunization, mice were challenged subcutaneously with 5×l05 MS+B16 cells. Compared with that of the negative control immunized with phosphate-buffered saline(PBS), a signi- ficant reduction of tumor growth was observed in groups immunized with survivin plasmid DNA and MUC1 VNTRs plasmid DNA. Thus, the suppression of subcutaneous tumor was antigen-specific. This model is useful for the development of tumor vaccines targeting survivin and MUCI VNTRs.

关键词: Survivin, MUC1 variable-number tandem repeat(MUC1 VNTR), Tumor antigen, Tumor model, Tumor vaccine

Abstract: The eukaryotic vectors VR1012 expressing survivin or 33 tandem repeats of human mucin 1(MUC1) (VNTRs), namely, VR1012-S and VR1012-VNTR(VNTR=variable number of tandem repeat), were constructed by cloning survivin and VNTR genes into VR1012, respectively. The eukaryotic vector pEGFP expressing survivin and MUC1 VNTRs fusion gene pEGFP-MS was also constructed. Mouse melanoma cell line(B16) stably expressing survivin and MUC1 VNTRs(MS+B16) was established by Lipofectamine-mediated transfection of pEGFP-MS into B16 cells. EGFP expression in MS+B16 cells was observed using a fluorescent microscope and survivin and MUC1 VNTRs(MS) expression was confirmed by means of Western blot analysis. A syngenic graft tumor model was gene- rated by subcutaneous injection of MS+B16 cells into C57/BL6 mice and tumor size increased rapidly with time in a cell number dependent manner. After the third immunization, mice were challenged subcutaneously with 5×l05 MS+B16 cells. Compared with that of the negative control immunized with phosphate-buffered saline(PBS), a signi- ficant reduction of tumor growth was observed in groups immunized with survivin plasmid DNA and MUC1 VNTRs plasmid DNA. Thus, the suppression of subcutaneous tumor was antigen-specific. This model is useful for the development of tumor vaccines targeting survivin and MUCI VNTRs.

Key words: Survivin, MUC1 variable-number tandem repeat(MUC1 VNTR), Tumor antigen, Tumor model, Tumor vaccine