Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (2): 353-357 .

Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

SUN Hui, CHEN Rui-chao, LI Ang, XU Guo-zhi

School of Materials Science and Mechanical Engineering, Beijing Technology and Business University, Beijing 100048, P. R. China

收稿日期:2011-07-01 修回日期:2011-09-10 出版日期:2012-03-25 发布日期:2012-03-08
通讯作者: SUN Hui E-mail:sunhui@th.btbu.edu.cn
基金资助:
Supported by the Scientific and Technological Development Programs of Beijing Municipal Education Commission, China (No.KM201010011004), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education, China, the 2011 Merit-based Research Funding for Picked Returned Overseas Scholars, Beijing Municipality(China) and the Research Project Approved for Graduate Students of Beijing Technology and Business University, China(No.19000101026).

Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

School of Materials Science and Mechanical Engineering, Beijing Technology and Business University, Beijing 100048, P. R. China

Received:2011-07-01 Revised:2011-09-10 Online:2012-03-25 Published:2012-03-08
Supported by:
Supported by the Scientific and Technological Development Programs of Beijing Municipal Education Commission, China (No.KM201010011004), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education, China, the 2011 Merit-based Research Funding for Picked Returned Overseas Scholars, Beijing Municipality(China) and the Research Project Approved for Graduate Students of Beijing Technology and Business University, China(No.19000101026).

摘要/Abstract

摘要： UV-induced graft polymerization of acrylic acid(AA) on poly(ether ether ketone)(PEEK) films was carried out to introduce ―COOH for the subsequent immobilization of bovine serum albumin(BSA). BSA was introduced on PEEK surface based on the condensation reaction between ―NH₂ and ―COOH. The modified surface (PEEK-BSA) was characterized by energy-disperse spectrometry(EDS), X-ray photoelectron spectroscopy(XPS), water contact angle measurement and UV spectrum analysis. The contact angle was found to decrease from 104° for the virgin PEEK films to 63° for the BSA-immobilized PEEK films, demonstrating a significant improvement of surface hydrophilicity. Moreover, the appearance of nitrogen on PEEK film confirmed by XPS and EDS indicates the immobilization of BSA on PEEK surface.

关键词: Poly(ether ether ketone)(PEEK), UV-grafting, Bovine serum albumin immobilization

Abstract: UV-induced graft polymerization of acrylic acid(AA) on poly(ether ether ketone)(PEEK) films was carried out to introduce ―COOH for the subsequent immobilization of bovine serum albumin(BSA). BSA was introduced on PEEK surface based on the condensation reaction between ―NH₂ and ―COOH. The modified surface (PEEK-BSA) was characterized by energy-disperse spectrometry(EDS), X-ray photoelectron spectroscopy(XPS), water contact angle measurement and UV spectrum analysis. The contact angle was found to decrease from 104° for the virgin PEEK films to 63° for the BSA-immobilized PEEK films, demonstrating a significant improvement of surface hydrophilicity. Moreover, the appearance of nitrogen on PEEK film confirmed by XPS and EDS indicates the immobilization of BSA on PEEK surface.

Key words: Poly(ether ether ketone)(PEEK), UV-grafting, Bovine serum albumin immobilization

SUN Hui, CHEN Rui-chao, LI Ang, XU Guo-zhi. Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement[J]. 高等学校化学研究, 2012, 28(2): 353-357 .

SUN Hui, CHEN Rui-chao, LI Ang, XU Guo-zhi. Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement[J]. Chemical Research in Chinese Universities, 2012, 28(2): 353-357 .

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Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

Immobilization of Bovine Serum Albumin on Poly(ether ether ketone) for Surface Biocompatibility Improvement

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