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高等学校化学研究 ›› 1998, Vol. 14 ›› Issue (4): 365-369.

• Articles • 上一篇    下一篇

Studies on Phosphate Effect of Cytosine and Its Novel Photoproduct

LIN Feng1, WANG Wen-qing2, WU Ji-lan2   

  1. 1. Department of Chemistry, Hangzhou University, Hangzhou 310028;
    2. Department of Technical Physics, Peking University, Beijing 100871
  • 收稿日期:1997-10-18 出版日期:1998-12-24 发布日期:2011-08-17
  • 通讯作者: LIN Feng
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.:29672003)and the Science Research Foundation of Hangzhou University(No.:9712098)..

Studies on Phosphate Effect of Cytosine and Its Novel Photoproduct

LIN Feng1, WANG Wen-qing2, WU Ji-lan2   

  1. 1. Department of Chemistry, Hangzhou University, Hangzhou 310028;
    2. Department of Technical Physics, Peking University, Beijing 100871
  • Received:1997-10-18 Online:1998-12-24 Published:2011-08-17

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被引次数

6

摘要： Under the irradiation of a middle pressure mercury lamp(MPML), we found that the photo lyses of nucleobases, nucleosides and nucleotides were strongly enhanced by phosphates (termed phosphate effect). In order to explain this phenomenon, the photolytic systems of cytosine in phosphate solutions were selected and studied. Experimental results indicated that the UV irradiation (190-220 nm) in the emission spectra of MPML is responsible for the phosphate effect. Phosphates absorbing the UV (190-220 nm) energy are converted to phosphate radical anions, which react with cytosine, leading to a novel photoproduct with phosphate group (C4H6N3O5P). It has been isolated, purified and characterized by means of elementary analysis, UV, IR, EI MS, 1H NMR, 13 C NMR and 31 P NMR. The phosphate effect and its formation mechanism have been discussed.

关键词: Phosphate effect, Photolysis of cytosine, Photoproduct 6-phosphocytosine

Abstract: Under the irradiation of a middle pressure mercury lamp(MPML), we found that the photo lyses of nucleobases, nucleosides and nucleotides were strongly enhanced by phosphates (termed phosphate effect). In order to explain this phenomenon, the photolytic systems of cytosine in phosphate solutions were selected and studied. Experimental results indicated that the UV irradiation (190-220 nm) in the emission spectra of MPML is responsible for the phosphate effect. Phosphates absorbing the UV (190-220 nm) energy are converted to phosphate radical anions, which react with cytosine, leading to a novel photoproduct with phosphate group (C4H6N3O5P). It has been isolated, purified and characterized by means of elementary analysis, UV, IR, EI MS, 1H NMR, 13 C NMR and 31 P NMR. The phosphate effect and its formation mechanism have been discussed.

Key words: Phosphate effect, Photolysis of cytosine, Photoproduct 6-phosphocytosine