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高等学校化学研究 ›› 1999, Vol. 15 ›› Issue (2): 116-121.

• Articles • 上一篇    下一篇

Cleavage of Nucleotides by Lanthanide Metal Complexes

ZHU Bing, ZHAO Da-qing, Ni Jia-zuan   

  1. Laboratory of Rare Earth Chemistry, Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, 130022
  • 收稿日期:1998-04-27 出版日期:1999-04-24 发布日期:2011-08-17
  • 通讯作者: ZHAO Da-qing
  • 基金资助:

    Supported by the National Program "The National Natural Science Foundation of China and The Basic Research of Rare Earth Science of China".

Cleavage of Nucleotides by Lanthanide Metal Complexes

ZHU Bing, ZHAO Da-qing, Ni Jia-zuan   

  1. Laboratory of Rare Earth Chemistry, Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, 130022
  • Received:1998-04-27 Online:1999-04-24 Published:2011-08-17
  • Supported by:

    Supported by the National Program "The National Natural Science Foundation of China and The Basic Research of Rare Earth Science of China".

PDF (PC)

摘要： The present work revealed that the praseodymium(Ⅲ) complex of 2-carboxyethylgermanium sesquioxide(Ge-132) promotes the hydrolysis of the phosphodiester linkages of 3',5'-cyclic adenosine monophosphate(cAMP), 3',5'-cyclic deoxyadenosine monophosphate(dcAMP), 5'-adenosine monophosphate(5'-AMP), 5'-deoxyadenosine monophosphate(5'-dAMP) under mild conditions. Both cAMP, dcAMP were hydrolyzed sitespecifically, yielding predominantly 3'-monophosphates, the main products of the cleavage of 5'-AMP, 5'-dAMP included adenosine(Ado), deoxyadenosine(dAdo), free phosphates respectively. A hydrolytic mechanism was proposed for cAMP, dcAMP, 5'-AMP, 5'-dAMP.

关键词: Lanthanide metal(Ⅲ) complexes, Nucleotides cleavage, Artificial nucleases

Abstract: The present work revealed that the praseodymium(Ⅲ) complex of 2-carboxyethylgermanium sesquioxide(Ge-132) promotes the hydrolysis of the phosphodiester linkages of 3',5'-cyclic adenosine monophosphate(cAMP), 3',5'-cyclic deoxyadenosine monophosphate(dcAMP), 5'-adenosine monophosphate(5'-AMP), 5'-deoxyadenosine monophosphate(5'-dAMP) under mild conditions. Both cAMP, dcAMP were hydrolyzed sitespecifically, yielding predominantly 3'-monophosphates, the main products of the cleavage of 5'-AMP, 5'-dAMP included adenosine(Ado), deoxyadenosine(dAdo), free phosphates respectively. A hydrolytic mechanism was proposed for cAMP, dcAMP, 5'-AMP, 5'-dAMP.

Key words: Lanthanide metal(Ⅲ) complexes, Nucleotides cleavage, Artificial nucleases