高等学校化学研究 ›› 1995, Vol. 11 ›› Issue (3): 215-218.
Studies on HEPES Stabilizing M Intermediate of Bacteriorhodopsin
FU Xue-qi1, LI Zheng-qiang2, WANG Jin-dui1, WANG BaO-huia3, LI Bo-fu2, LI Tie-jin3
- 1. National Key Laboratory of Enzyme Engineering;
2. Key Laboratory of Molecular Spectrum and Molecular Structure;
3. Department of Chemistry, Jilin University, Changchun, 130023
Studies on HEPES Stabilizing M Intermediate of Bacteriorhodopsin
FU Xue-qi1, LI Zheng-qiang2, WANG Jin-dui1, WANG BaO-huia3, LI Bo-fu2, LI Tie-jin3
- 1. National Key Laboratory of Enzyme Engineering;
2. Key Laboratory of Molecular Spectrum and Molecular Structure;
3. Department of Chemistry, Jilin University, Changchun, 130023
摘要： HEPES has proved to stabilize Mintermediate of Bacteriorhodopsin.Absorp-tion and Raman spectra showed that there was a more stable form of Mintermedi-ate in BR-HEPES solution.It was found by SPSthat there were two main interme-diates .M and O, existing in BR-HEPES solution.