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高等学校化学研究 ›› 1990, Vol. 6 ›› Issue (4): 295-299.

• Articles • 上一篇    下一篇

Biosensors Based on the Tissue of Plant as Catalytic Material for Amino Acids

Qi Deyao1, Zhuang Yunlong1, Chi Qijin2   

  1. 1. Department of Chemistry and Chemical Engineering, Shanghai University of Technology, Shanghai;
    2. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun
  • 收稿日期:1990-09-17 出版日期:1990-12-24 发布日期:2011-09-09

Biosensors Based on the Tissue of Plant as Catalytic Material for Amino Acids

Qi Deyao1, Zhuang Yunlong1, Chi Qijin2   

  1. 1. Department of Chemistry and Chemical Engineering, Shanghai University of Technology, Shanghai;
    2. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun
  • Received:1990-09-17 Online:1990-12-24 Published:2011-09-09

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摘要： Two tissue electrodes are described in this paper. It is shown that a portion of intact tissue from apple or cactus can be coupled with potentiometric ammonia gas sensing electrode to prepare sensors for some amino acids with good response properties. Their optimum operating conditions and properties with respect to buffer sort, pHof the bulk solution, selectivity, linearity, stability, sensitivity and response time are studied and discussed. At the same time, the dynamic parameters, such as Km and Vm, of L-glutamine deaminasc and L-as-paragine deamlnase have been obtained by means of these tissue electrodes.

关键词: Biosensors, Tissue electrodes, Biocatalysis, Apple, Cactus

Abstract: Two tissue electrodes are described in this paper. It is shown that a portion of intact tissue from apple or cactus can be coupled with potentiometric ammonia gas sensing electrode to prepare sensors for some amino acids with good response properties. Their optimum operating conditions and properties with respect to buffer sort, pHof the bulk solution, selectivity, linearity, stability, sensitivity and response time are studied and discussed. At the same time, the dynamic parameters, such as Km and Vm, of L-glutamine deaminasc and L-as-paragine deamlnase have been obtained by means of these tissue electrodes.

Key words: Biosensors, Tissue electrodes, Biocatalysis, Apple, Cactus