[an error occurred while processing this directive]

高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (2): 134-138.

• Articles • 上一篇    下一篇

Statistical Optimization of Medium Components for Improved Product Ion of Recombinant Hyperthermophilic Esterase

REN Xiao-dong, YU Da-wei, HAN Si-ping, FENG Yan   

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, P.R.China
  • 收稿日期:2006-01-10 出版日期:2006-04-24 发布日期:2011-08-06
  • 基金资助:

    Supported by the 973 Project(Nos.2003CB716000 and 2004CB719606).

Statistical Optimization of Medium Components for Improved Product Ion of Recombinant Hyperthermophilic Esterase

REN Xiao-dong, YU Da-wei, HAN Si-ping, FENG Yan   

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, P.R.China
  • Received:2006-01-10 Online:2006-04-24 Published:2011-08-06
  • Supported by:

    Supported by the 973 Project(Nos.2003CB716000 and 2004CB719606).

PDF (PC)

摘要： The optimization of nutrient levels for the production of recombinant hyperthermophilic esterase by E.coli was carried out with response surface methodology(RSM) based on the central composite rotatable design(CCRD).A 24central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals.The P-value of the coefficient for the linear effect of peptone concentration was 0.0081 and trace metals solution was less than 0.0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production.The predicted optimal hyperthermophilic esterase yield was 269.17 U/mL, whereas an actual experimental value of 284.58 U/mL was obtained.

关键词: Optimization, Medium components, Recombinant hyperthermophilic esterase and response surface meth-odology

Abstract: The optimization of nutrient levels for the production of recombinant hyperthermophilic esterase by E.coli was carried out with response surface methodology(RSM) based on the central composite rotatable design(CCRD).A 24central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals.The P-value of the coefficient for the linear effect of peptone concentration was 0.0081 and trace metals solution was less than 0.0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production.The predicted optimal hyperthermophilic esterase yield was 269.17 U/mL, whereas an actual experimental value of 284.58 U/mL was obtained.

Key words: Optimization, Medium components, Recombinant hyperthermophilic esterase and response surface meth-odology