Synthesis of Highly ordered Large Size Mesoporous Silica and Effect of Stabilization as Enzyme Supports in Organic Solvent

摘要/Abstract

摘要： FSM-16, MCM-41 and SBA-15 types of hexagonal mesoporous silica with a highly ordered 2-dimensional structure were synthesized by using different silicon sources and surfactants. In the 2-dimensional silicate framework, pore size can be uniformly controlled by the combined use of the surfactants having different alkyl chain lengths and the swelling agents(triisopropyl benzene). The pore-diameter of FSM-16 and MCM-41 can be expanded to be 10 nm, SBA-15 to be 15 nm. The crystal regularity was decreased with the increase of the porediameter. In FSM-16 derived from kanemite (silicon source) and MCM-41 from water glass, their anionic characteristics on the pore-wall may be stronger than those of SBA-15 derived from oligomeric tetramethoxysilane(TMOS). We have successfully used FSM-16 and MCM-41 to immobilize the enzyme having cationic residues below isoelectric point. The level of adsorption of enzymes in FSM-16 and MCM-41 was relatively high, but was low in SBA-15 support. The mechanism of enzyme to be adsorbed in mesoporous silica was suggested to be the ionic interactions. In aqueous solutions, horseradish peroxidase(HTP) was immobilized in FSM-16 with 8. 9 nm mesopores and the highest loading amount (183 mg/mg FSM) was obtained, but for the FSM-16 of pore diameter 30 nm only an amount of 28 mg/mg FSM was obtained. The catalytic activity in the organic solvent was high when HRP was immobilized in FSM-16 and MCM-41, but it was low in case of SBA-15.

关键词: Mesoporous silica, Surfactant, Swelling agent, Enzyme, Activity

Abstract: FSM-16, MCM-41 and SBA-15 types of hexagonal mesoporous silica with a highly ordered 2-dimensional structure were synthesized by using different silicon sources and surfactants. In the 2-dimensional silicate framework, pore size can be uniformly controlled by the combined use of the surfactants having different alkyl chain lengths and the swelling agents(triisopropyl benzene). The pore-diameter of FSM-16 and MCM-41 can be expanded to be 10 nm, SBA-15 to be 15 nm. The crystal regularity was decreased with the increase of the porediameter. In FSM-16 derived from kanemite (silicon source) and MCM-41 from water glass, their anionic characteristics on the pore-wall may be stronger than those of SBA-15 derived from oligomeric tetramethoxysilane(TMOS). We have successfully used FSM-16 and MCM-41 to immobilize the enzyme having cationic residues below isoelectric point. The level of adsorption of enzymes in FSM-16 and MCM-41 was relatively high, but was low in SBA-15 support. The mechanism of enzyme to be adsorbed in mesoporous silica was suggested to be the ionic interactions. In aqueous solutions, horseradish peroxidase(HTP) was immobilized in FSM-16 with 8. 9 nm mesopores and the highest loading amount (183 mg/mg FSM) was obtained, but for the FSM-16 of pore diameter 30 nm only an amount of 28 mg/mg FSM was obtained. The catalytic activity in the organic solvent was high when HRP was immobilized in FSM-16 and MCM-41, but it was low in case of SBA-15.

Key words: Mesoporous silica, Surfactant, Swelling agent, Enzyme, Activity

LI Bo, Inagaki S., Miyazaki C., Takahashi H.. Synthesis of Highly ordered Large Size Mesoporous Silica and Effect of Stabilization as Enzyme Supports in Organic Solvent[J]. 高等学校化学研究, 2002, 18(2): 200-205.

LI Bo, Inagaki S., Miyazaki C., Takahashi H.. Synthesis of Highly ordered Large Size Mesoporous Silica and Effect of Stabilization as Enzyme Supports in Organic Solvent[J]. Chemical Research in Chinese Universities, 2002, 18(2): 200-205.

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