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高等学校化学研究 ›› 2002, Vol. 18 ›› Issue (3): 294-298.

• Articles • 上一篇    下一篇

Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures

GOU Xiao-jun1, LI Chun-lei1, KONG Xiang-duo1, ZHU Shi-zhen1, ZHANG Hong-ying1, WANG Xiao-ping1, ZHANG Jin1, LIU Tian-yun2   

  1. 1. Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, P. R. China;
    2. The People's Hospital of Tongjiang Country, Tongjiang 635700, P. R. China
  • 收稿日期:2001-07-25 出版日期:2002-08-24 发布日期:2011-08-04

Directed Evolution of Human Cu,Zn-SOD Under Artificial Oxidant Pressures

GOU Xiao-jun1, LI Chun-lei1, KONG Xiang-duo1, ZHU Shi-zhen1, ZHANG Hong-ying1, WANG Xiao-ping1, ZHANG Jin1, LIU Tian-yun2   

  1. 1. Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, P. R. China;
    2. The People's Hospital of Tongjiang Country, Tongjiang 635700, P. R. China
  • Received:2001-07-25 Online:2002-08-24 Published:2011-08-04

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摘要： Cu,Zn-SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn-SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn-SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.

关键词: Human Cu, Zn-SOD, Directed evolution, Screening

Abstract: Cu,Zn-SOD is a highly conserved enzyme and the controversy about its evolutionary possibility in the near future has been lively. In order to further our understanding of the future fate of human Cu,Zn-SOD, we adopted a strategy relating to the directed evolution to study how the mutants of human Cu,Zn-SOD respond to different oxidative stress. After five rounds of screening, we found a mutant that can survive under harsh pressures and DNA sequencing proves that it shows a mutation responsible for the phenomenon. However, under natural pressure, our screening comes to nothing. Then we may draw the following conclusions: the evolution of biological macromolecules in some respect depends on their surroundings and if they are too familiar with a certain environment, they may embody evolutionary inertia.

Key words: Human Cu,Zn-SOD, Directed evolution, Screening