Single-Molecule FRET Study of DNA G-Quadruplex

高等学校化学研究 ›› 2002, Vol. 18 ›› Issue (2): 103-106.

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Single-Molecule FRET Study of DNA G-Quadruplex

Jeremy Green, Li ming Ying, David Klenerman, Shankar Blasubramanian

Department of Chemistry, University of Cambridge, Lens field Road, Cambridge CB2 1EW, UK

收稿日期:2001-12-10 出版日期:2002-04-24 发布日期:2011-08-04
基金资助:
Supported by the BBSRC.

Single-Molecule FRET Study of DNA G-Quadruplex

Jeremy Green, Li ming Ying, David Klenerman, Shankar Blasubramanian

Department of Chemistry, University of Cambridge, Lens field Road, Cambridge CB2 1EW, UK

Received:2001-12-10 Online:2002-04-24 Published:2011-08-04
Supported by:
Supported by the BBSRC.

摘要/Abstract

摘要： The DNA G-quadruplex formed by the human telomeric sequence is a potential target for novel anticancer drugs. We have investigated an intramolecular DNA G-quadruplex using single-molecule fluorescence resonance energy transfer and shown that individual folded quadruplexes can be identified. The mean proximity ratio measured at the single-molecule level was consistent with ensemble measurement.

关键词: G-quadruplex, Fluorescence resonance energy transfer(FRET), Single-molecule spectroscopy

Abstract: The DNA G-quadruplex formed by the human telomeric sequence is a potential target for novel anticancer drugs. We have investigated an intramolecular DNA G-quadruplex using single-molecule fluorescence resonance energy transfer and shown that individual folded quadruplexes can be identified. The mean proximity ratio measured at the single-molecule level was consistent with ensemble measurement.

Key words: G-quadruplex, Fluorescence resonance energy transfer(FRET), Single-molecule spectroscopy

Jeremy Green, Li ming Ying, David Klenerman, Shankar Blasubramanian. Single-Molecule FRET Study of DNA G-Quadruplex[J]. 高等学校化学研究, 2002, 18(2): 103-106.

Jeremy Green, Li ming Ying, David Klenerman, Shankar Blasubramanian. Single-Molecule FRET Study of DNA G-Quadruplex[J]. Chemical Research in Chinese Universities, 2002, 18(2): 103-106.

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Single-Molecule FRET Study of DNA G-Quadruplex

Single-Molecule FRET Study of DNA G-Quadruplex

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