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高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (3): 413-416.

• Articles • 上一篇    下一篇

Effects of PAHs on Biotransformation Enzymatic Activities in Fish

LU Guang-hua, CHEN Wei*, LI Ying and ZHU Zhi   

  1. Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, P. R. China
  • 收稿日期:2010-06-02 修回日期:2010-09-28 出版日期:2011-05-25 发布日期:2011-04-29
  • 通讯作者: CHEN Wei E-mail:cw5826@hhu.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.51079049) and the Crucial Special Project of National Water Pollution Control and Management Science of China(No.2008ZX07421-002).

Effects of PAHs on Biotransformation Enzymatic Activities in Fish

LU Guang-hua, CHEN Wei*, LI Ying and ZHU Zhi   

  1. Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, P. R. China
  • Received:2010-06-02 Revised:2010-09-28 Online:2011-05-25 Published:2011-04-29
  • Contact: CHEN Wei E-mail:cw5826@hhu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.51079049) and the Crucial Special Project of National Water Pollution Control and Management Science of China(No.2008ZX07421-002).

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被引次数

16

摘要： Biotransformation and detoxification responses to the exposure to five polycyclic aromatic hydrocarbons were investigated in crucian(Carassius auratus). Juvenile crucian were treated with a single intraperitoneal injection of each compound at dosages of 0.1, 1.0, 2.0, 5.0 and 8.0(or 10.0) mg/kg and sacrificed 15 d later to determine 7-ethoxyresorufin-O-deethylase(EROD) and glutathione S-transferases(GST) activities in gill S9 fractions. EROD activity is significantly increased by benzo(b)fluoranthene and indeno(1,2,3-cd)pyrene at all the doses. High dosages of PAHs induced GST activity and the inducing ability of them increased in the following order: fluorene<      fluoranthene<indeno(1,2,3-cd)pyrene<benzo(g,h,i)perylene<benzo(b)fluoranthene. In all the cases, dose dependence appeared to exist. The gill EROD and GST in Carassius auratus are useful biomarkers to estimate sub-acute toxicity of both polycyclic aromatic hydrocarbons(PAHs) and PAHs-like compounds.

关键词: Polycyclic aromatic hydrocarbon, Gill, 7-Ethoxyresorufin-O-deethylase activity, Glutathione S-transfe- rases activity

Abstract: Biotransformation and detoxification responses to the exposure to five polycyclic aromatic hydrocarbons were investigated in crucian(Carassius auratus). Juvenile crucian were treated with a single intraperitoneal injection of each compound at dosages of 0.1, 1.0, 2.0, 5.0 and 8.0(or 10.0) mg/kg and sacrificed 15 d later to determine 7-ethoxyresorufin-O-deethylase(EROD) and glutathione S-transferases(GST) activities in gill S9 fractions. EROD activity is significantly increased by benzo(b)fluoranthene and indeno(1,2,3-cd)pyrene at all the doses. High dosages of PAHs induced GST activity and the inducing ability of them increased in the following order: fluorene<      fluoranthene<indeno(1,2,3-cd)pyrene<benzo(g,h,i)perylene<benzo(b)fluoranthene. In all the cases, dose dependence appeared to exist. The gill EROD and GST in Carassius auratus are useful biomarkers to estimate sub-acute toxicity of both polycyclic aromatic hydrocarbons(PAHs) and PAHs-like compounds.

Key words: Polycyclic aromatic hydrocarbon, Gill, 7-Ethoxyresorufin-O-deethylase activity, Glutathione S-transfe- rases activity